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Synergistic control of auxin mediated transcription by ARF and C/S1 bZIP transcription factors

ARF 和 C/S1 bZIP 转录因子对生长素介导的转录的协同控制

基本信息

批准号：
17280683
负责人：
Professor Dr. Wolfgang Dröge-Laser
金额：
--
依托单位：
Lehrstuhl für Pharmazeutische Biologie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2005
资助国家：
德国
起止时间：
2004-12-31 至 2019-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/17280683?language=en
关键词：
Synergistic control auxin mediated transcription

项目摘要

In order to sustain optimal plant growth, auxin regulated transcription is adjusted according to environ-mental signals. This project studies the integration of signals into auxin-mediated transcriptional pat-terns. Using the Arabidopsis GH3.3 promoter as a model, we could demonstrate that AUXIN RESPONSE ELEMENTS (AuxREs), G-BOX RELATED ELEMENTS (GREs) and MYB RESPONSIVE ELEMENTS (MREs) cooperate in auxin-mediated transcription. Whereas AUXIN RESPONSE FACTORS (ARFs) mediate auxin-responsiveness via AuxREs, GREs and MREs act as quantitative elements. This project focuses on the impact of the group S1 basic leucine zipper (bZIP) transcription factors AtbZIP2, AtbZIP11 and AtbZIP44 which modulate auxin responses via binding to GREs. Estra-diol-induced gain- and loss-of function approaches in transgenic plants will be used to study the impact of these bZIPs on auxin-regulated transcription and its in vivo binding to the respective promoters. De-pending on bZIP expression, typical auxin responses such as initiation of lateral roots, root hair forma-tion and gravitropic responses will be studied. As C/S1 bZIP network modulates primary metabolism in response to energy stress, we postulate that the GRE/bZIP module functions as a “rheostate” which provides means to balance auxin-mediated growth responses with the energy status of the cell.

为了维持植物的最佳生长，生长素调节的转录根据环境信号进行调节。本项目研究生长素介导的转录模式中信号的整合。以拟南芥GH3.3启动子为模型，我们可以证明生长素响应元件（AuxREs）、G-BOX相关元件（GREs）和MYB响应元件（MREs）在生长素介导的转录中协同作用。生长素反应因子（ARFs）通过AuxREs介导生长素反应，而GREs和MREs则作为定量因子。本项目重点研究S1组基本亮氨酸拉链（bZIP）转录因子AtbZIP2、AtbZIP11和AtbZIP44通过与GREs结合调节生长素反应的影响。在转基因植物中，利用雌二醇诱导的功能增益和功能丧失方法来研究这些bzip对生长素调控的转录及其在体内与各自启动子结合的影响。根据bZIP的表达，将研究典型的生长素反应，如侧根的形成、根毛的形成和向地性反应。由于C/S1 bZIP网络调节初级代谢以应对能量应激，我们假设GRE/bZIP模块作为一种“流变状态”，提供了平衡生长素介导的生长反应和细胞能量状态的手段。