SGER: Electrophile Responsive Proteome and Their Role in Plant Signaling Pathways

SGER：亲电响应蛋白质组及其在植物信号通路中的作用

• 批准号: 0606838
• 负责人: Katayoon Dehesh
• 金额: --
• 依托单位: University of California-Davis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-01-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0606838&HistoricalAwards=false
• 关键词: SGER; Electrophile; Responsive; Proteome; Their

Various environmental and developmental stimuli generate reactive electrophile species (RES) that modify proteins through covalent adduct formation and trigger adaptive cellular responses in animal systems. Although this post-translational modification termed electrophile mediated thiol-binding (EMTB) is known to regulate signal transduction pathways in animals, it has not been previously reported in plant systems. Recently it was discovered that EMTB modification of a key enzyme in the oxylipin pathway of plants functions as an important signal in plant stress responses. The goal of this project is to examine the general role of EMTB modification in controlling plant signaling pathways.Intellectual Merit: Interdisciplinary approaches will be used to identify, on a genome-wide scale, the cellular target proteins that are covalently modified by stress-induced 12-oxophytodienoic acid (12-OPDA), a lipid RES in plants. A subset of the 12-OPDA binding protein targets will be fully characterized and examined for their role in plant stress signaling pathways. Finally, the specificity of 12-OPDA versus the other stress-induced thiolreactive electrophiles in covalent modification of the proteins identified will be determined. Broader impacts: The results of this study will provide important foundational insights into a novel, previously unrecognized posttranslational regulatory mechanism controlling a complex and crucial signaling network in plants with significant relevance to animal signaling systems. In addition the discovery of pathway components regulated by ETMB may provide for the generation of tools for biotechnological applications in agronomically important crops. This project will also provide for the training of undergraduate students and postdoctoral fellows in state-of-the-art interdisciplinary research and will include efforts to recruit underrepresented minority undergraduate students, the broad dissemination of research results in papers, seminars, and conference presentations, and the development of short courses on the role of post-translational modification in signaling pathways.

各种环境和发育刺激会产生反应性亲电子物质 (RES)，这些物质通过共价加合物形成来修饰蛋白质，并触发动物系统中的适应性细胞反应。尽管已知这种被称为亲电子介导的硫醇结合（EMTB）的翻译后修饰可以调节动物中的信号转导途径，但之前在植物系统中尚未有报道。 最近发现，EMTB对植物氧脂素途径中关键酶的修饰是植物逆境反应中的重要信号。该项目的目标是研究 EMTB 修饰在控制植物信号通路中的一般作用。 智力优点：将使用跨学科方法在全基因组范围内识别由胁迫诱导的 12-氧代植物二烯酸 (12-OPDA)（植物中的一种脂质 RES）共价修饰的细胞靶蛋白。 12-OPDA 结合蛋白靶标的子集将得到全面表征并检查其在植物胁迫信号传导途径中的作用。 最后，将确定 12-OPDA 相对于其他应激诱导的硫醇反应性亲电子试剂在所鉴定蛋白质的共价修饰中的特异性。 更广泛的影响：这项研究的结果将为了解一种新颖的、以前未被认识的翻译后调节机制提供重要的基础见解，该机制控制植物中复杂而关键的信号网络，与动物信号系统具有显着相关性。 此外，受 ETMB 调节的途径成分的发现可能会为农艺重要作物的生物技术应用提供工具的产生。 该项目还将为本科生和博士后研究员提供最先进的跨学科研究方面的培训，包括努力招募代表性不足的少数族裔本科生，在论文、研讨会和会议演讲中广泛传播研究成果，以及开发关于信号通路中翻译后修饰作用的短期课程。