Orthogonal Modification of Biopolymers with Variable Peptide Sequences via Ultra-Rapid Covalent Modification

通过超快速共价修饰对具有可变肽序列的生物聚合物进行正交修饰

• 批准号: 175717199
• 负责人: Professor Dr. Christopher Barner-Kowollik
• 金额: --
• 依托单位: Institut für Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/175717199?language=en
• 关键词: Orthogonal; Modification; Biopolymers; Variable; Peptide

In the present proposal, ultra-rapid ambient temperature and catalyst-free coupling chemistry is investigated to be employed to bind monomer sequence-defined peptides onto biopolymer based surfaces. The aim will be to ultimately develop an access route to peptide modified biosubstrates, where the peptide segments constitute functions such as specific cell adhesion, biocidal activity or modulation of crystallization processes. The current proposal will demonstrate that extremely mild and catalyst-free conjugation strategies are ideally suited to ligate sensitive biomolecules to biological substrates.The overall aim of the project is subdivided into individual work packages, which have well-defined aims of their own. These are (i) the pre-functionalization and characterization of variable poly(saccharide) surfaces, e.g. cellulose, chitin, chitosan and hyaluronic acid systems, with highly electron deficient dithioesters, (ii) the establishment of a synthetic methodology to introduce dithioester or cyclopentadienyl moieties to supported peptides including model studies of the coupling of these in homogenous solution to synthetic polymers or soluble poly(saccharides), (iii) the functionalization of the electron deficient dithioester decorated poly(saccharide) surfaces with peptide sequences as well as the in-depth characterization of the generated surfaces, and (iv) the application of the developed ultra-rapid ambient temperature grafting procedure for the preparation of functional substrates.

在本提案中，研究了超快速环境温度和无催化剂的偶联化学，以用于将单体序列限定的肽结合到基于生物聚合物的表面上。目标将是最终开发肽修饰的生物底物的进入途径，其中肽片段构成诸如特异性细胞粘附、杀生物活性或结晶过程的调节的功能。目前的提案将证明，极其温和和无催化剂的缀合策略非常适合将敏感的生物分子连接到生物底物上。该项目的总体目标被细分为单独的工作包，这些工作包都有自己明确的目标。这些是（i）可变聚的预官能化和表征（ii）建立了一种合成方法，将二硫酯或二硫酯部分引入到负载的肽中，包括在均相溶液中将这些偶联到合成聚合物或可溶性聚（三）的功能化的缺电子的二硫代酯修饰的聚（糖）表面与肽序列，以及在所产生的表面的深入表征，和（iv）的应用程序开发的超快速环境温度接枝程序的功能基板的制备。