Cellular localization and signal transduction of a novel family of olfactory receptors

新型嗅觉受体家族的细胞定位和信号转导

• 批准号: 177981225
• 负责人: Professorin Dr. Sigrun Korsching
• 金额: --
• 依托单位: Work Group Neurobiology
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/177981225?language=en
• 关键词: Cellular; localization; signal; transduction; novel

The activation of olfactory receptors by odors constitutes the first step in the neuronal processing of olfactory stimuli. The subsequent signal transduction cascade is characteristically different for the different families of olfactory receptor genes and determines the processing properties of the corresponding olfactory receptor neurons. We have identified a novel family of olfactory receptors, the ORAs, which are GPCRs and related to the mammalian VIR pheromone receptor gene family. The ORA family exhibits several novel and unexpected properties and is therefore of high interest.With this application we wish to determine, which of the three known types of olfactory receptor neurons expresses the ora genes. Using co-localization studies we will find out, which G proteins and ion channels may contribute to signal transduction of ORA receptors. Competition and deprivation experiments together with activation of receptors by ligands will prove the contribution of candidate signal transduction molecules. We will genetically label the ORA-expressing receptor neurons in zebrafish to determine their target glomeruli.

气味激活嗅觉受体是神经元处理嗅觉刺激的第一步。对于不同家族的嗅觉受体基因，随后的信号转导级联具有不同的特征，并决定了相应嗅觉受体神经元的处理特性。我们已经鉴定出一个新的嗅觉受体家族，即 ORA，它们是 GPCR，与哺乳动物 VIR 信息素受体基因家族相关。 ORA家族表现出一些新颖和意想不到的特性，因此引起了高度关注。通过该应用，我们希望确定三种已知类型的嗅觉受体神经元中的哪一种表达ora基因。通过共定位研究，我们将发现哪些 G 蛋白和离子通道可能有助于 ORA 受体的信号转导。竞争和剥夺实验以及配体激活受体将证明候选信号转导分子的贡献。我们将对斑马鱼中表达 ORA 的受体神经元进行基因标记，以确定其目标肾小球。

项目成果

A single identified glomerulus in the zebrafish olfactory bulb carries the high-affinity response to death-associated odor cadaverine

• DOI: 10.1038/srep40892
• 发表时间: 2017-01-19
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: Dieris, Milan;Ahuja, Gaurav;Korsching, Sigrun I.
• 通讯作者: Korsching, Sigrun I.