Nuclear Magnetic Resonance Spectroscopy on a Chip

芯片上的核磁共振波谱分析

• 批准号: 0622228
• 负责人: Axel Scherer
• 金额: $ 30万
• 依托单位: California Institute of Technology
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0622228&HistoricalAwards=false
• 关键词: Nuclear; Magnetic; Resonance; Spectroscopy; Chip

This proposal focuses on the integration of novel micrometer and nanometer scale Nuclear Magnetic Resonance (NMR) devices and materials into the lithographically fabricated micro-fluidic systems. Such miniaturization and implementation of NMR systems on a micro-fluidic platform will lead to the highly parallel chemical analysis of smaller volumes of bio-chemically important solutions with significantly greater sensitivity than previously possible. Reducing the size of Magnetic Resonance Imaging (MRI) devices on a chip will also lead to the non-destructive imaging of biological cells with an unprecedented spatial resolution.Intellectual Merit Micro-fluidic chip-based NMR systems will be designed and fabricated implementing (a) components for application of large gradient magnetic fields, (b) permanent magnet-based devices that provide high local fields, and (c) micro/nanometer scale electro-magnetic coils that will significantly improve the sensitivity and resolution of NMR and MRI. This research will offer new tools for the study of individual cells, enabling both spectroscopic and imaging observation of changes in cell metabolism triggered by the micro-fluidic controlled environmental changes. Furthermore, the micro-fluidic NMR chips will provide the ability to manipulate pico-liters of solution and perform highly parallel NMR biochemical analysis. Fundamental limits of miniaturization and integration of NMR micro-technologies within the elastomeric micro-fluidic chips will also be explored.Broader Impact This program will foster collaboration between a Ph.D. granting institution (Caltech) and a large, urban, minority serving non-Ph.D. granting university (CSULB). The Ph.D. and Masters students from both institutions will be exposed to the multi-disciplinary techniques from engineering, physics, and biochemistry, while being trained in micro-fabrication, fluidics, sensor design, and magnetic resonance imaging and spectroscopy techniques. The research that students perform is expected to find many other applications where small samples have to be analyzed and imaged within an inexpensive portable chip-based platform with particular emphasis towards disease diagnostics.

该提案的重点是将新型微米和纳米级核磁共振（NMR）器件和材料集成到光刻制造的微流体系统中。在微流体平台上的NMR系统的这种小型化和实施将导致对较小体积的生物化学重要溶液的高度并行化学分析，其灵敏度比以前可能的高得多。减小芯片上磁共振成像（MRI）设备的尺寸也将导致以前所未有的空间分辨率对生物细胞进行非破坏性成像。智能优点基于微流控芯片的NMR系统将设计和制造实现（a）用于大梯度磁场应用的组件，（B）提供高局部场的基于永磁体的设备，以及（c）将显著提高NMR和MRI的灵敏度和分辨率的微/纳米级电磁线圈。 这项研究将为单个细胞的研究提供新的工具，使光谱和成像观察由微流体控制的环境变化引发的细胞代谢变化成为可能。此外，微流控NMR芯片将提供操作皮升溶液和执行高度并行NMR生化分析的能力。还将探索弹性体微流控芯片中核磁共振微技术小型化和集成的基本限制。更广泛的影响该计划将促进博士之间的合作授予机构（加州理工学院）和一个大的，城市，少数服务非博士。授予大学（CSULB）。的博士来自两个机构的硕士生将接触到工程，物理和生物化学的多学科技术，同时接受微制造，流体，传感器设计，磁共振成像和光谱技术的培训。学生们进行的研究预计将发现许多其他应用，其中小样本必须在廉价的便携式基于芯片的平台内进行分析和成像，特别强调疾病诊断。