Momentum transfer through synthesized biopolymer network meshes with optically trapped anchor points

通过具有光学捕获锚点的合成生物聚合物网络网格进行动量传递

• 批准号: 179729698
• 负责人: Professor Dr. Alexander Rohrbach
• 金额: --
• 依托单位: Institut für Mikrosystemtechnik (IMTEK)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/179729698?language=en
• 关键词: Momentum; transfer; through; synthesized; biopolymer

During the last two decades, optical traps have proven their enormous potential in soft matter physics and in biophysics. Although nearly arbitrary potential landscapes can be generated quite flexibly, particle motions therein and driving forces are often difficult to measure. Only briefly it has been shown that by fast time-multiplexing of an optical trap, parallel tracking of about 100 particles is possible in three dimensions achieving a rate of several kHz and nanometer precision. An independent calibration of all point traps allows for measuring dynamic force fields with high precision and sensitivity.The generation and measurement of arbitrary force fields makes it possible to investigate new intelligent, soft materials based on the model of nature. In this proposal we aim to set up a network of biopolymer through a bottom-up approach, where each elementary cell is set up by optical traps to be connected with the next cell. Microtubules, which are an important part of the cytoskeleton inside living cells, will be bound in chemically stabilized variant to coated latex beads. Each bead sits inside an optical trap within a variable two-dimensional arrangement and serves as an anchor point of the network. By disturbing the network mechanically and at different frequencies at one end, the propagation of momentum and the visco-elastic response transferred by different mesh configurations can be studied at the other end.

在过去的二十年中，光学陷阱证明了它们在软物质物理和生物物理学中的巨大潜力。尽管可以非常灵活地产生几乎任意的潜在景观，但是其中的粒子运动和驱动力通常很难测量。仅简短地表明，通过对光学陷阱的快速时间进行交流，在三个维度上可以平行跟踪约100个颗粒，从而达到几个KHz和纳米精度的速率。对所有点陷阱的独立校准允许以高精度和敏感性来测量动态力场。任意力场的生成和测量使得根据自然模型研究了新的智能，软材料。在此提案中，我们旨在通过自下而上的方法建立一个生物聚合物网络，在该方法中，每个基本单元均由光学陷阱建立，以与下一个单元格连接。微管是活细胞内细胞骨架的重要组成部分，将与化学稳定变体结合到涂层的乳胶珠。每个珠子都位于变量二维布置中的光学陷阱内，并用作网络的锚点。通过机械地和一端的不同频率在不同的频率上打扰网络，可以在另一端研究动量的传播和通过不同网格配置传输的粘弹性响应。