Momentum transfer through synthesized biopolymer network meshes with optically trapped anchor points

通过具有光学捕获锚点的合成生物聚合物网络网格进行动量传递

• 批准号: 179729698
• 负责人: Professor Dr. Alexander Rohrbach
• 金额: --
• 依托单位: Institut für Mikrosystemtechnik (IMTEK)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2010
• 资助国家: 德国
• 起止时间: 2009-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/179729698?language=en
• 关键词: Momentum; transfer; through; synthesized; biopolymer

During the last two decades, optical traps have proven their enormous potential in soft matter physics and in biophysics. Although nearly arbitrary potential landscapes can be generated quite flexibly, particle motions therein and driving forces are often difficult to measure. Only briefly it has been shown that by fast time-multiplexing of an optical trap, parallel tracking of about 100 particles is possible in three dimensions achieving a rate of several kHz and nanometer precision. An independent calibration of all point traps allows for measuring dynamic force fields with high precision and sensitivity.The generation and measurement of arbitrary force fields makes it possible to investigate new intelligent, soft materials based on the model of nature. In this proposal we aim to set up a network of biopolymer through a bottom-up approach, where each elementary cell is set up by optical traps to be connected with the next cell. Microtubules, which are an important part of the cytoskeleton inside living cells, will be bound in chemically stabilized variant to coated latex beads. Each bead sits inside an optical trap within a variable two-dimensional arrangement and serves as an anchor point of the network. By disturbing the network mechanically and at different frequencies at one end, the propagation of momentum and the visco-elastic response transferred by different mesh configurations can be studied at the other end.

在过去的二十年中，光阱已经证明了它们在软物质物理和生物物理中的巨大潜力。虽然可以相当灵活地生成几乎任意的潜在景观，但其中的粒子运动和驱动力通常难以测量。仅简要地表明，通过光阱的快速时分复用，可以在三维中并行跟踪约100个颗粒，实现几kHz的速率和纳米精度。所有点阱的独立校准允许以高精度和高灵敏度测量动态力场。任意力场的生成和测量使得研究基于自然模型的新型智能软材料成为可能。在这个提议中，我们的目标是通过自下而上的方法建立一个生物聚合物网络，其中每个基本细胞都由光阱建立，与下一个细胞连接。微管是活细胞内细胞骨架的重要组成部分，将以化学稳定的变体结合到涂覆的乳胶珠上。每个珠子位于可变二维排列内的光阱内，并用作网络的锚点。通过在一端以不同的频率机械地扰动网络，可以在另一端研究由不同的网格配置传递的动量的传播和粘弹性响应。