CAREER:Genome-wide Analysis of Pathogen-induced Endogenous siRNAs in Plant Defense Responses in Arabidopsis

职业生涯：拟南芥植物防御反应中病原体诱导的内源 siRNA 的全基因组分析

• 批准号: 0642843
• 负责人: Hailing Jin
• 金额: $ 74.57万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-15 至 2013-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0642843&HistoricalAwards=false
• 关键词: CAREER; Genome; wide; Analysis; Pathogen

RNA silencing, mediated by small interfering RNAs (siRNAs), has evolved as an antiviral defense mechanism in plants and animals. However, it is not known whether host cells also utilize siRNAs in antibacterial and antifungal defenses. Thousands of endogenous siRNAs have been discovered by deep sequencing recently, but their important function in gene regulation has just started to be unveiled. This project will identify endogenous siRNAs that are induced by bacterial and fungal pathogens on a genome-wide scale and characterize the biogenesis and mode of action of a subset of the siRNAs. Furthermore, the physiological functions of these siRNAs and their target genes in plant defense responses will be examined. This project explores a previously uncharted area in plant defense responses. This work will likely reveal new regulatory mechanisms underlying plant disease resistance and defense responses and ultimately contribute to the development of new strategies to combat plant pathogens to improve agriculture. As an important component of this project, an innovative summer course with a follow-up training program is designed for undergraduate students from UC Riverside (UCR) and a local Hispanic Serving Institution San Bernardino Valley College. This summer course will cover basic concepts and provide hands-on experience in the area of Plant Molecular Biology and siRNA-Mediated Gene Silencing. A follow-up training program will be provided to those students who have interest in research. This program will mainly target women and minority students who have no or limited access to research opportunities, with the aim of enhancing their chance of pursuing advanced education and a scientific career in the future.

RNA沉默是一种由小干扰RNA （sirna）介导的抗病毒防御机制，已在植物和动物中得到发展。然而，宿主细胞是否也利用sirna进行抗菌和抗真菌防御尚不清楚。近年来，深度测序已经发现了数千种内源性sirna，但它们在基因调控中的重要功能才刚刚被揭示出来。该项目将在全基因组范围内鉴定由细菌和真菌病原体诱导的内源性sirna，并表征其中一部分sirna的生物发生和作用模式。此外，这些sirna及其靶基因在植物防御反应中的生理功能将被进一步研究。该项目探索了植物防御反应中一个以前未知的领域。这项工作可能会揭示植物抗病和防御反应的新调控机制，并最终有助于制定对抗植物病原体的新策略，以改善农业。作为该项目的一个重要组成部分，为加州大学河滨分校（UCR）和当地的西班牙裔服务机构圣贝纳迪诺山谷学院的本科生设计了一个具有后续培训计划的创新暑期课程。本暑期课程将涵盖植物分子生物学和sirna介导的基因沉默领域的基本概念和实践经验。对于那些对研究感兴趣的学生，将提供后续培训计划。该项目主要针对没有或有限的研究机会的妇女和少数民族学生，旨在增加他们将来接受高等教育和从事科学事业的机会。