Physical Chemistry of Macromolecules as Probed by High Resolution Solid State NMR

高分辨率固态核磁共振探测大分子的物理化学

• 批准号: 0718796
• 负责人: Kurt Zilm
• 金额: $ 50.43万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-01 至 2010-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0718796&HistoricalAwards=false
• 关键词: Physical; Chemistry; Macromolecules; Probed; Resolution

In this award, funded by the Experimental Physical Chemistry Program of the Chemistry Division, Professor Kurt Zilm of Yale University and his graduate and undergraduate research students will continue experiments to develop novel Nuclear Magnetic Resonance (NMR) methods. The proposed work is aimed at developing new NMR tools to monitor chemical events in proteins and to determine their structures using submicron sized crystals. Four model proteins and several isotope labeled peptides will serve as platforms for the investigation of new methods including NMR relaxation as a means for determining very long distances, the design of novel low electric field NMR resonators, and development of new sample spinning technology. The proposed investigations will expand the biophysical chemistry toolbox for the study of important macromolecular systems. This work should impact areas ranging from the design of chemical sensors, chemical catalysis, self-assembly of biomaterials, and protein misfolding. Along with producing students who will be instrument building experimentalists at the interface of biology and chemistry, Professor Zilm is actively involved in the recruitment of underrepresented groups in natural science research through the Yale University Science and Technology Research Scholars (STARS) program.

该奖项由化学学部实验物理化学项目资助，耶鲁大学的Kurt Zilm教授和他的研究生和本科生将继续实验开发新的核磁共振（NMR）方法。这项工作旨在开发新的核磁共振工具来监测蛋白质中的化学事件，并使用亚微米大小的晶体来确定它们的结构。四种模型蛋白和几种同位素标记肽将作为研究新方法的平台，包括核磁共振弛豫作为确定超长距离的手段，新型低电场核磁共振谐振器的设计，以及新样品旋转技术的开发。所提出的研究将扩展生物物理化学工具箱，用于研究重要的大分子系统。这项工作将影响化学传感器的设计、化学催化、生物材料的自组装和蛋白质错误折叠等领域。除了培养学生成为生物学和化学领域的仪器制造实验员外，Zilm教授还通过耶鲁大学科学技术研究学者（STARS）项目积极参与招募自然科学研究中代表性不足的群体。