PHYSICAL CHEMISTRY OF BIOLOGICAL MACROMOLECULES

生物大分子物理化学

• 批准号: 3896167
• 负责人: M S LEWIS
• 金额: --
• 依托单位: NATIONAL CENTER FOR RESEARCH RESOURCES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3896167
• 关键词: actins; analytical ultracentrifugation; arginase; cytokine receptors; enzyme mechanism; enzyme structure; interleukin 2; keratin; macromolecule; mathematical model; microtubules; molecular biology; molecular size; molecular weight; nucleotides; protein glutamine gamma glutamyltransferase; protein structure function; tubulin

The purpose of this project is to study the physical properties of a wide variety of biological macromolecules with the goal of correlating these properties to the structure and function of the macromolecules. The emphasis is on the thermodynamics of the interactions of these macromolecules and on their molecular size and shape. Analytical ultracentrifugation and mathematical modeling are the principal research techniques used. Studies on actin have been directed toward investigating the mechanisms involved in actin polymerization and the interaction of actin with actobindin. Studies on epidermal transglutaminase have involved characterization of the proenzyme form and study of the mechanism of activation. Studies on yeast arginase have been directed toward studying the reversible homogeneous association of this enzyme and the role of metal ions in controlling this. Studies of various deoxynucleotides have been directed toward investigating the reversible monomer-dimer equilibrium as a function of structure. Studies on alpha-helical fragments of keratin have been directed toward elucidating their role in keratin filaments. Studies on interleukin-2 receptors have been directed toward an elucidiction of the structure of the molecule. Studies on trypanosomatid proteins and tubulin and microtubules have been directed to an understanding of their interaction.

这个项目的目的是研究一种广泛的 各种生物大分子，目的是将它们相互关联 性质与大分子的结构和功能有关。这个 重点放在这些物质相互作用的热力学上。 大分子以及它们的分子大小和形状。分析性 主要研究内容为超速离心法和数学模型法 使用的技术。 对肌动蛋白的研究主要是为了研究其作用机制。 参与肌动蛋白聚合及其与肌动蛋白的相互作用 Actobindin。表皮谷氨酰胺转氨酶的研究已经涉及到 酶原形态的表征及其作用机理的研究 激活。酵母精氨酸酶的研究正朝着研究的方向发展 该酶的可逆均相结合与金属的作用 离子在控制这方面的作用。各种脱氧核苷酸的研究已经被 旨在研究可逆单体-二聚体平衡作为一种 结构的功能。角蛋白α-螺旋片段的研究 旨在阐明它们在角蛋白细丝中的作用。研究 关于白细胞介素2受体的研究已被用于阐明 分子结构。锥虫蛋白和微管蛋白的研究 而微管已经被引导到理解它们的 互动。