Physical Chemistry of Macromolecules as Probed by High Resolution Solid State NMR

高分辨率固态核磁共振探测大分子的物理化学

• 批准号: 1012573
• 负责人: Kurt Zilm
• 金额: $ 50万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2014-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1012573&HistoricalAwards=false
• 关键词: Physical; Chemistry; Macromolecules; Probed; Resolution

In this project, funded by the Chemical Measurement and Imaging Program of the Chemistry Division, Professor Kurt Zilm at Yale University will develop novel Nuclear Magnetic Resonance (NMR) techniques to study the physical chemistry of large molecules in a site-specific manner. Advances in dynamic nuclear polarization (DNP), which enhances NMR signals by over 100-fold, will be adapted to microliter size samples. This has the potential to make feasible biophysical studies on recombinant membrane proteins with as little as 100 nanograms of material. Other methods will be developed to determine distances the order of 10 Angstroms by simple NMR relaxation measurements. These techniques will be applied to understand how mutations in visual pigments change the structure of the retinal chromophore, and for observing the dynamics of buried water molecules in proteins. Broader impacts of this research include expanding the biophysical chemistry toolbox, and the training of students as instrument building experimentalists at the interface of biology and chemistry. Professor Zilm is actively involved in the recruitment of underrepresented students in natural science research through the Yale University Science and Technology Research Scholars program, and as the host of chemistry laboratory open houses for local high school classes.

在这个由化学部化学测量和成像计划资助的项目中，耶鲁大学的Kurt Zilm教授将开发新型核磁共振（NMR）技术，以特定的方式研究大分子的物理化学。动态核极化（DNP）的进展，提高了100倍以上的NMR信号，将适应微升大小的样品。 这有可能使可行的生物物理研究重组膜蛋白与少至100纳克的材料。将开发其他方法，通过简单的NMR弛豫测量来确定10埃量级的距离。这些技术将被应用于了解视觉色素的突变如何改变视网膜发色团的结构，以及观察蛋白质中埋藏的水分子的动态。这项研究的更广泛的影响，包括扩大生物物理化学工具箱，并在生物和化学的接口的仪器建设实验员的学生的培训。Zilm教授通过耶鲁大学科学技术研究学者计划积极参与自然科学研究中代表性不足的学生的招聘，并担任当地高中班级化学实验室开放日的主持人。