PHYSICAL CHEMISTRY OF BIOLOGICAL MACROMOLECULES

生物大分子物理化学

• 批准号: 3959941
• 负责人: M S LEWIS
• 金额: --
• 依托单位: DIVISION OF RESEARCH SERVICES
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3959941
• 关键词: analytical ultracentrifugation; antifibrinolytic agents; blood coagulation; cell adhesion; chemical structure function; fibrinogen; fibrinolysis; macromolecule; mathematical model; molecular biology; molecular size; molecular weight; monoclonal antibody; plasminogen; platelet activating factor; synapses; thermodynamics

The purpose of this project is to study the physical properties of a wide variety of biological macromolecules with the goal of correlating these properties to the structure and function of the macromolecules. The emphasis is on the thermodynamics of the interactions of these macromolecules and on their molecular size and shape. Analytical ultracentrifugation and mathematical modeling are the principal research techniques used. Studies on the association of fibrinogen with other proteins involved in blood clotting and fibrinolysis have been continued. Studies in progress in this area deal with the association of fibrinogen with plasma and platelet Factor XIII and with the association of plasminogen with the D and E fragments of fibrinogen. Research on ricin has involved studies on a monoclonal antibody to the ricin B chain, the interaction of this antibody with ricin, and its effect on ricin toxicity both in vitro and in vivo. Studies on synapsin have been directed toward determining the nature of the reactions involved in the self-association of this protein.

该项目的目的是研究一种 各种各样的生物大分子， 将这些性质与药物的结构和功能相关联， 大分子 重点是在热力学的 这些大分子的相互作用及其分子大小 和形状。 分析超浓缩和数学 建模是主要的研究技术。 纤维蛋白原与其他蛋白质结合的研究 参与血液凝固和纤维蛋白溶解的研究仍在继续。 这一领域正在进行的研究涉及 纤维蛋白原与血浆和血小板因子XIII和与 纤溶酶原与纤溶酶D和E片段的结合 纤维蛋白原。 对蓖麻毒素的研究包括对单克隆抗体的研究 与蓖麻毒素B链的相互作用， 及其对蓖麻毒素毒性的影响。 对突触蛋白的研究一直致力于确定 这种自缔合反应的性质 蛋白