Chemical Vapor Deposition Fabrication of Nano- and Micro-scale Biomimetic Surfaces

纳米和微米级仿生表面的化学气相沉积制造

• 批准号: 0727984
• 负责人: Rebecca Carrier
• 金额: --
• 依托单位: Northeastern University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-10-01 至 2011-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0727984&HistoricalAwards=false
• 关键词: Chemical; Vapor; Deposition; Fabrication; Nano

The objective of this proposed research is to develop an intestinal cell culture substrate to improve the accuracy of in vitro drug transport studies and provide a scaffold for intestinal tissue regeneration. A culture substrate that exactly mimics the natural topography of intestinal basement membrane will be developed, providing essential developmental cues to cells cultured in vitro. Chemical vapor deposition (CVD) will be used to create a silica template of intestinal basement membrane that exactly mimics the native nano- and micro-topography. CVD will be used to caoat the silica mold with a porous biocompatible polymer such as poly(2-hydroxyethyl methacrylate) (pHEMA). This polymer substrate will be separated from the silica mold and used for cell culture. SEM will be used to characterize the silica and pHEMA topology and compare it to native issue. The influence of the topography of the developed device on enterocyte cell phenotype and transport properties will be compared with cells cultured on non-topographically modified surfaces via a variety of assays.This work will result in the development of a cell-based tool for studying transport across the intestinal epithelium, leading to cost and time savings in drug studies, as well as a fundamental understanding of cell-biomaterial interactions and native intestinal function in normal and disease (e.g. colon cancer) states. If successful, this research will significantly reduce the time and development costs for bringing pharmaceuticals to market, as well as make serious contributions to the treatment of numerous intestinal illnesses, including short bowel syndrome, Crohn's disease, and colon cancer. It offers a greatly improved approach to current cell culture techniques through the creation of culture substrates that exactly mimic important features found in vivo, and provides basic scientific insight into the influence of nano- and micro-topography on enterocyte phenotype.

本研究的目的是开发一种肠细胞培养基质，以提高体外药物转运研究的准确性，并为肠组织再生提供支架。将开发一种完全模拟肠基底膜天然形貌的培养基质，为体外培养的细胞提供必要的发育线索。化学气相沉积（CVD）将被用来创建一个二氧化硅模板的肠基底膜，完全模仿天然的纳米和微观形貌。CVD将用于用多孔生物相容性聚合物如聚（甲基丙烯酸2-羟乙酯）（pHEMA）涂覆二氧化硅模具。将该聚合物基质与二氧化硅模具分离并用于细胞培养。SEM将用于表征二氧化硅和pHEMA拓扑结构，并将其与天然组织进行比较。将通过各种试验将所开发的装置的形貌对肠上皮细胞表型和转运特性的影响与在非形貌修饰表面上培养的细胞进行比较。这项工作将导致开发用于研究跨肠上皮转运的基于细胞的工具，从而节省药物研究的成本和时间，以及对细胞-生物材料相互作用和正常和疾病（例如结肠癌）状态下的天然肠道功能的基本理解。如果成功，这项研究将大大减少将药物推向市场的时间和开发成本，并为治疗许多肠道疾病做出重大贡献，包括短肠综合征，克罗恩病和结肠癌。它提供了一个大大改进的方法，目前的细胞培养技术，通过创建培养基质，完全模仿体内发现的重要特征，并提供了基本的科学见解的影响，纳米和微观形貌肠上皮细胞表型。