MRI: Acquisition of a Flow Cytometer 4 Color Dual Laser Basic for the Identification and Quantification of Cells

位于明尼苏达州圣克劳德的圣克劳德州立大学(SCSU)获得了一笔赠款，以获得一种多功能且易于使用的仪器，可以执行快速、多色的流式细胞仪分析，以检测和量化不同类型的细胞。智能优势：流式细胞仪将为南加州大学带来一种高度灵敏的工具，对免疫学、遗传学、生物化学、微生物学、寄生虫学、生理学、生理学和水产养殖等领域的复杂细胞和分子分析至关重要。华南理工大学生物系和化学系的九个研究实验室都需要识别和量化不同的细胞分子。该仪器的获得将使PI能够阐明免疫反应信号转导中的重要生物学机制。此外，流式细胞仪还将用于研究真核细胞的转录调控、细胞周期控制、肿瘤细胞敏感性、激素反应、细菌毒素活性和环境水污染。因此，流式细胞仪的使用将促进九个实验室的多样化研究项目，以及普通细胞分析技术的本科生研究培训。更广泛的影响：将建议的仪器纳入本科课程也将极大地改进几门生物和化学课程的实验室实践，包括免疫学、血液学和细胞培养技术。一台坚固、多功能、多色的流式细胞仪是华南理工大学等研究和教学机构的基本设备。它的收购不仅将丰富初级研究、跨部门合作和本科教学，而且还将提高华南理工大学对新生和潜在教师的吸引力。

A grant has been awarded to St. Cloud State University (SCSU), St. Cloud, Minnesota, to acquire a flow cytometer that is a versatile and easy-to-use instrument that can perform quick, multicolor flow cytometric analysis in order to detect and quantify different types of cells. Intellectual Merit: The flow cytometer will bring to SCSU a highly sensitive tool crucial for sophisticated cellular and molecular analysis in areas such as immunology, genetics, biochemistry, microbiology, parasitology, physiology, phycology and aquatics. Nine research laboratories in the Biology and Chemistry departments at SCSU all require identification and quantification of different cell molecules. The acquisition of the proposed instrument will allow the PI to elucidate important biological mechanisms involved in signal transduction of immune responses. In addition, the flow cytometer will be used to research eukaryotic transcriptional regulation, cell cycle control, tumor cell sensitivity, hormonal responses, bacterial toxin activity, and environmental aquatic contamination. Thus, the utilization of the flow cytometer will promote diverse research projects by nine laboratories, and undergraduate research training in common cell analysis technique. Broader Impacts: Incorporation of proposed instrument into undergraduate curricula will also greatly improve the laboratory practices of several biology and chemistry classes, including Immunology, Hematology, and Cell culture techniques. A robust, versatile, multicolor flow cytometer is a fundamental piece of equipment for a research and teaching institution such as SCSU. Its acquisition will not only enrich primary research, cross-departmental collaboration and undergraduate teaching, but also promote attractiveness of SCSU to new prospective students and faculty.