MRI: Acquisition of a Flow Cytometer 4 Color Dual Laser Basic for the Identification and Quantification of Cells

MRI：获取流式细胞仪 4 色双激光基本型，用于细胞识别和定量

• 批准号: 0821235
• 负责人: Marina Cetkovic-Cvrlje
• 金额: --
• 依托单位: St. Cloud State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-01 至 2011-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0821235&HistoricalAwards=false
• 关键词: MRI; Acquisition; Flow; Cytometer; Color

A grant has been awarded to St. Cloud State University (SCSU), St. Cloud, Minnesota, to acquire a flow cytometer that is a versatile and easy-to-use instrument that can perform quick, multicolor flow cytometric analysis in order to detect and quantify different types of cells. Intellectual Merit: The flow cytometer will bring to SCSU a highly sensitive tool crucial for sophisticated cellular and molecular analysis in areas such as immunology, genetics, biochemistry, microbiology, parasitology, physiology, phycology and aquatics. Nine research laboratories in the Biology and Chemistry departments at SCSU all require identification and quantification of different cell molecules. The acquisition of the proposed instrument will allow the PI to elucidate important biological mechanisms involved in signal transduction of immune responses. In addition, the flow cytometer will be used to research eukaryotic transcriptional regulation, cell cycle control, tumor cell sensitivity, hormonal responses, bacterial toxin activity, and environmental aquatic contamination. Thus, the utilization of the flow cytometer will promote diverse research projects by nine laboratories, and undergraduate research training in common cell analysis technique. Broader Impacts: Incorporation of proposed instrument into undergraduate curricula will also greatly improve the laboratory practices of several biology and chemistry classes, including Immunology, Hematology, and Cell culture techniques. A robust, versatile, multicolor flow cytometer is a fundamental piece of equipment for a research and teaching institution such as SCSU. Its acquisition will not only enrich primary research, cross-departmental collaboration and undergraduate teaching, but also promote attractiveness of SCSU to new prospective students and faculty.

已向明尼苏达州圣克劳德的圣克劳德州立大学（SCSU）提供了一笔赠款，用于购买一台流式细胞仪，这是一种通用且易于使用的仪器，可以进行快速、快速的流式细胞仪分析，以检测和量化不同类型的细胞。智力优势：流式细胞仪将为SCSU带来一种高度敏感的工具，对于免疫学、遗传学、生物化学、微生物学、寄生虫学、生理学、藻类学和水产学等领域的复杂细胞和分子分析至关重要。SCSU生物学和化学系的九个研究实验室都需要对不同的细胞分子进行鉴定和定量。 收购拟议的仪器将允许PI阐明参与免疫反应信号转导的重要生物学机制。此外，流式细胞仪还将用于研究真核生物的转录调控、细胞周期控制、肿瘤细胞敏感性、激素反应、细菌毒素活性和环境水污染。因此，流式细胞仪的使用将促进九个实验室的多样化研究项目，以及普通细胞分析技术的本科生研究培训。 更广泛的影响：将拟议的仪器纳入本科课程也将大大提高几个生物和化学类，包括免疫学，血液学和细胞培养技术的实验室实践。一个强大的，多功能的，可拆卸的流式细胞仪是一个基本的设备，为研究和教学机构，如SCSU。 它的收购不仅将丰富初级研究，跨部门合作和本科教学，而且还将促进SCSU对新的潜在学生和教师的吸引力。