CAREER:Tailored Glycoconjugates for the Precise Detection of Toxins and Pathogens

职业：用于精确检测毒素和病原体的定制糖复合物

• 批准号: 0845005
• 负责人: James Mack II
• 金额: $ 57.5万
• 依托单位: University of Cincinnati Main Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0845005&HistoricalAwards=false
• 关键词: CAREER; Tailored; Glycoconjugates; Precise; Detection

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).The Organic and Macromolecular Chemistry Program in the Chemistry Division at the National Science Foundation supports Professor Suri S. Iyer at the University of Cincinnati who will synthesize ligands that exhibit antibody-like selectivity, are robust, inexpensive, amenable to scale up and require no refrigeration. Glycans possess these desirable properties and they are unique recognition molecules that modulate essential communication processes such as cell adhesion, proliferation, migration, and differentiation. However, synthetic glycoconjugates because of selectivity and sensitivity issues cannot be used for practical applications. A better understanding of the binding event at the molecular level will help to fill the gap in knowledge that has prevented the use of synthetic glycans in diagnostics and therapeutics. Consequently, the PI proposes to identify the molecular determinants (such as structure, multivalency and mode of presentation) of glycan-receptor interactions by developing a library of chemically defined glycans and assaying them with different strains of toxins and pathogens using a variety of biophysical techniques. The overall goal of the proposal is to understand how modifications in structure, valency and architecture modulate glycan-receptor recognition events. The successful outcome of this project will advance knowledge in the area of glycoscience and pathogen detection. It is expected that researchers will use the knowledge gained to rationally design ligands to modulate glycan mediated biochemical processes.The proposed activities will broaden the participation of underrepresented high school students by providing them with an opportunity to work in a research laboratory. Professor Iyer will provide an active and structured learning environment and will provide students with valuable training in multiple disciplines that include glycoscience, biophysical measurements and microbiology. In addition, a summer workshop for K-12 science teachers to promote training and exposure to emerging interdisciplinary areas will be included. It is expected that these K-12 teachers will be able expose students to these topics at an early age. The PI will continue to disseminate his research on glycoscience, biodetection, and nanoscience through publications, reviews, presentations, and visits to local schools and rotary clubs.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。他将合成具有抗体样选择性的配体，这种配体坚固耐用，价格低廉，易于放大，不需要冷藏。聚糖具有这些所需的性质，并且它们是调节基本通信过程（例如细胞粘附、增殖、迁移和分化）的独特识别分子。然而，由于选择性和敏感性问题，合成的糖缀合物不能用于实际应用。在分子水平上更好地理解结合事件将有助于填补知识上的差距，这阻碍了在诊断和治疗中使用合成聚糖。因此，PI建议通过开发化学定义的聚糖库并使用各种生物物理技术用不同的毒素和病原体菌株对其进行测定，来鉴定聚糖-受体相互作用的分子决定因素（如结构、多价性和呈递模式）。该提案的总体目标是了解结构，化合价和结构的修饰如何调节聚糖受体识别事件。该项目的成功结果将推进糖科学和病原体检测领域的知识。预计研究人员将利用所获得的知识来合理设计配体，以调节聚糖介导的生化过程。拟议的活动将扩大代表性不足的高中生的参与，为他们提供在研究实验室工作的机会。Iyer教授将提供一个积极和结构化的学习环境，并将为学生提供包括糖科学，生物物理测量和微生物学在内的多个学科的宝贵培训。此外，还将为K-12科学教师举办暑期讲习班，以促进培训和接触新兴的跨学科领域。预计这些K-12教师将能够让学生在很小的时候就接触到这些主题。PI将继续通过出版物、评论、演讲和访问当地学校和扶轮社来传播他在糖科学、生物检测和纳米科学方面的研究。