CAREER: Biomolecular Nanophotonic Fabry-Perot Interferometry (BioNanoFPI)

职业：生物分子纳米光子法布里-珀罗干涉仪 (BioNanoFPI)

• 批准号: 0845370
• 负责人: Long Que
• 金额: $ 40万
• 依托单位: Louisiana Tech University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2014-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0845370&HistoricalAwards=false
• 关键词: CAREER; Biomolecular; Nanophotonic; Fabry; Perot

CAREER: Biomolecular Nanophotonic Fabry-Perot Interferometry (BioNanoFPI)The proposes to design a Biomolecular Nanophotonic Fabry-Perot Interferometer (BioNanoFPI) platform that will allow scalable parallel detection of multiple bio-agents with concentrations in the femtomole range and ease-of-operation using a broadband white light source instead of a laser. The development of a polymer-based micromachined FPI (µFPI) with integrated nanostructures will enable a BioNanoFPI platform that allows for the creation of two-dimensional, highly-multiplexed, inexpensive arrays to conduct large-scale parallel screening of chemical and biomedical libraries.The objective of this CAREER project is to establish a significant advancement in biomolecular nanophotonics by theoretical modeling and interfacing liquid-state biopolymers to solid-state nanophotonic and micromachined devices. There are three main components of this career development plan. The scientific component of this project focuses on: (i) the theoretical modeling and understanding of and experimental confirmation of the signal enhancement mechanism of the nanopillar plasmonic substrates, and the interactions between the nanopillar-plasmonic substrates and micromachined Fabry-Perot Interferometry (µFPI); (ii) the theoretical modeling of the dielectric behavior of biopolymers and experimental elucidation of interactions of antibodies and antigens in the nanostructured Fabry-Perot cavity. The technological component of this project focuses on: (i) the development of an inexpensive nanofabrication process to construct a nanopillar array for plasmonic substrates; (ii) the precise control of sub100 nm nanostructure/nanopillar arrays in the µFPI cavity for highly sensitive label free bioassays, and a robust batch fabrication method of nanostructure-filled polymer-based µFPI arrays integrated with micro and nanofluidic networks. Intellectual merit: This proposed research will help advance fundamental knowledge of signal enhancement mechanisms of the nanopillar plasmonic substrates and the BioNanoFPI micro/nanosystem. Understanding the fundamental physical mechanism of this micro/nanosystem might trigger other important ideas and innovations for bionanotechnology applications. This research has a broad range of applications to pathogen, disease detection, environmental monitoring and security. In addition, drug screening and discovery can benefit tremendously by using high throughput multiplexed label-free biosensing.Broader impacts: The PI proposes a coherent and comprehensive education, dissemination and outreach component that includes developing a new technical elective course ?Introduction to Nano-biophotonics,? integrating research results with existing nano and micro courses, mentoring graduate, undergraduate and underrepresented students and dissemination and outreach to the local community. A webpage will be designed especially to disseminate the outcomes of ?Introduction to Nano-biophotonics.? The proposed educational and outreach program will be accomplished through the NSF-sponsored REU program at Louisiana Tech to educate undergraduate students and through the NSF-sponsored NERO program to educate women and under-represented students in K-12, high school and prospective students from local small rural and small town schools. The overall educational goal is to help next-generation workforce development by training students to carry out research with sound theory and allowing them to gain hands-on laboratory skills for their advanced careers.

职业：生物分子纳米光子法布里-珀罗干涉仪（BioNanoFPI）建议设计一个生物分子纳米光子法布里-珀罗干涉仪（BioNanoFPI）平台，该平台将允许使用宽带白光源而不是激光对浓度在飞摩尔范围内的多种生物制剂进行可扩展的并行检测，并且易于操作。具有集成纳米结构的基于聚合物的微机械 FPI (μFPI) 的开发将使 BioNanoFPI 平台成为可能，该平台允许创建二维、高度多重、廉价的阵列，以对化学和生物医学库进行大规模并行筛选。该职业项目的目标是通过理论建模在生物分子纳米光子学方面取得重大进展 以及将液态生物聚合物与固态纳米光子和微机械设备连接。该职业发展计划由三个主要组成部分组成。该项目的科学部分侧重于：（i）纳米柱等离子体基板的信号增强机制的理论建模、理解和实验确认，以及纳米柱等离子体基板与微机械法布里-珀罗干涉仪（μFPI）之间的相互作用； (ii) 生物聚合物介电行为的理论模型以及纳米结构法布里-珀罗腔中抗体和抗原相互作用的实验阐明。该项目的技术部分侧重于：（i）开发一种廉价的纳米加工工艺来构建用于等离子体基底的纳米柱阵列； (ii) µFPI 腔中亚 100 nm 纳米结构/纳米柱阵列的精确控制，用于高灵敏度无标记生物测定，以及与微纳流体网络集成的基于纳米结构填充聚合物的 µFPI 阵列的稳健批量制造方法。智力价值：这项研究将有助于增进纳米柱等离子体基底和 BioNanoFPI 微/纳米系统信号增强机制的基础知识。了解这种微/纳米系统的基本物理机制可能会引发生物纳米技术应用的其他重要想法和创新。这项研究在病原体、疾病检测、环境监测和安全方面具有广泛的应用。此外，通过使用高通量多重无标记生物传感，药物筛选和发现可以受益匪浅。更广泛的影响：PI提出了一个连贯且全面的教育、传播和推广部分，其中包括开发一门新技术选修课程“纳米生物光子学概论”。将研究成果与现有的纳米和微米课程相结合，指导研究生、本科生和代表性不足的学生，并向当地社区传播和推广。将专门设计一个网页来传播“纳米生物光子学导论”的成果。拟议的教育和推广计划将通过 NSF 资助的路易斯安那理工学院 REU 项目来完成，以教育本科生，并通过 NSF 资助的 NERO 项目来教育 K-12 中的女性和代表性不足的学生、高中以及当地农村和小镇学校的未来学生。总体教育目标是通过培训学生利用合理的理论进行研究并让他们获得实验室实践技能以促进其高级职业发展，从而帮助下一代劳动力发展。