CAREER: Biomolecular Nanophotonic Fabry-Perot Interferometry (BioNanoFPI)

职业：生物分子纳米光子法布里-珀罗干涉仪 (BioNanoFPI)

• 批准号: 1461841
• 负责人: Long Que
• 金额: $ 12.84万
• 依托单位: Iowa State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2015-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1461841&HistoricalAwards=false
• 关键词: CAREER; Biomolecular; Nanophotonic; Fabry; Perot

CAREER: Biomolecular Nanophotonic Fabry-Perot Interferometry (BioNanoFPI)The proposes to design a Biomolecular Nanophotonic Fabry-Perot Interferometer (BioNanoFPI) platform that will allow scalable parallel detection of multiple bio-agents with concentrations in the femtomole range and ease-of-operation using a broadband white light source instead of a laser. The development of a polymer-based micromachined FPI (µFPI) with integrated nanostructures will enable a BioNanoFPI platform that allows for the creation of two-dimensional, highly-multiplexed, inexpensive arrays to conduct large-scale parallel screening of chemical and biomedical libraries.The objective of this CAREER project is to establish a significant advancement in biomolecular nanophotonics by theoretical modeling and interfacing liquid-state biopolymers to solid-state nanophotonic and micromachined devices. There are three main components of this career development plan. The scientific component of this project focuses on: (i) the theoretical modeling and understanding of and experimental confirmation of the signal enhancement mechanism of the nanopillar plasmonic substrates, and the interactions between the nanopillar-plasmonic substrates and micromachined Fabry-Perot Interferometry (µFPI); (ii) the theoretical modeling of the dielectric behavior of biopolymers and experimental elucidation of interactions of antibodies and antigens in the nanostructured Fabry-Perot cavity. The technological component of this project focuses on: (i) the development of an inexpensive nanofabrication process to construct a nanopillar array for plasmonic substrates; (ii) the precise control of sub100 nm nanostructure/nanopillar arrays in the µFPI cavity for highly sensitive label free bioassays, and a robust batch fabrication method of nanostructure-filled polymer-based µFPI arrays integrated with micro and nanofluidic networks. Intellectual merit: This proposed research will help advance fundamental knowledge of signal enhancement mechanisms of the nanopillar plasmonic substrates and the BioNanoFPI micro/nanosystem. Understanding the fundamental physical mechanism of this micro/nanosystem might trigger other important ideas and innovations for bionanotechnology applications. This research has a broad range of applications to pathogen, disease detection, environmental monitoring and security. In addition, drug screening and discovery can benefit tremendously by using high throughput multiplexed label-free biosensing.Broader impacts: The PI proposes a coherent and comprehensive education, dissemination and outreach component that includes developing a new technical elective course ?Introduction to Nano-biophotonics,? integrating research results with existing nano and micro courses, mentoring graduate, undergraduate and underrepresented students and dissemination and outreach to the local community. A webpage will be designed especially to disseminate the outcomes of ?Introduction to Nano-biophotonics.? The proposed educational and outreach program will be accomplished through the NSF-sponsored REU program at Louisiana Tech to educate undergraduate students and through the NSF-sponsored NERO program to educate women and under-represented students in K-12, high school and prospective students from local small rural and small town schools. The overall educational goal is to help next-generation workforce development by training students to carry out research with sound theory and allowing them to gain hands-on laboratory skills for their advanced careers.

职业：生物分子纳米光子法布里-珀罗干涉仪（BioNanoFPI）提出设计一种生物分子纳米光子法布里-珀罗干涉仪（BioNanoFPI）平台，该平台将允许使用宽带白色光源而不是激光器对浓度在飞摩尔范围内的多种生物制剂进行可扩展的并行检测，并且易于操作。具有集成纳米结构的基于聚合物的微加工FPI（µFPI）的开发将实现BioNanoFPI平台，该平台允许创建二维、高度多路复用的该CAREER项目的目标是通过理论建模和将液态生物聚合物与固态生物聚合物相结合，所述纳米光子和微机械装置。该职业发展计划有三个主要组成部分。该项目的科学部分侧重于：（i）对纳米柱等离子体基片的信号增强机制以及纳米柱等离子体基片与微机械法布里-珀罗干涉仪（µFPI）之间的相互作用进行理论建模、理解和实验确认;（二）生物聚合物的介电行为的理论建模和抗体和抗原相互作用的实验阐明，纳米结构法布里-珀罗腔。该项目的技术组成部分集中在：（i）开发一种廉价的纳米纤维工艺，以构建等离子体基片的纳米柱阵列;（ii）精确控制µFPI腔中的亚100 nm纳米结构/纳米柱阵列，用于高灵敏度的无标记生物测定，以及集成微纳米流体网络的基于纳米结构填充聚合物的µFPI阵列的稳健批量制造方法。智力优点：这项研究将有助于推进纳米柱等离子体基片和BioNanoFPI微/纳米系统的信号增强机制的基础知识。了解这种微/纳米系统的基本物理机制可能会引发生物纳米技术应用的其他重要想法和创新。这项研究在病原体、疾病检测、环境监测和安全方面有着广泛的应用。此外，药物筛选和发现可以受益巨大，通过使用高通量多路复用无标记biosensing.Broader影响：PI提出了一个连贯和全面的教育，传播和推广的组成部分，包括开发一个新的技术选修课程？纳米生物光子学导论将研究成果与现有的纳米和微型课程相结合，指导研究生、本科生和代表性不足的学生，并向当地社区传播和推广。将专门设计一个网页，以传播？纳米生物光子学导论拟议的教育和推广计划将通过NSF赞助的路易斯安那理工大学REU计划来完成，以教育本科生，并通过NSF赞助的NERO计划来教育K-12，高中和未来的女性和代表性不足的学生来自当地小农村和小城镇学校。总体教育目标是通过培训学生以合理的理论进行研究，并让他们获得实践实验室技能，以帮助下一代劳动力的发展。