Functional analysis of Arabidopsis NSC1/DMI1 Ca2+ permeable channels

拟南芥 NSC1/DMI1 Ca2 通透通道的功能分析

• 批准号: 0848263
• 负责人: Zhen-Ming Pei
• 金额: $ 52.5万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-07-01 至 2013-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0848263&HistoricalAwards=false
• 关键词: Functional; analysis; Arabidopsis; NSC1; DMI1

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).PI name: Zhen-Ming PeiProject no. IOS-0848263Project title: Functional analysis of Arabidopsis NSC1 Ca2+ permeable channelsIntellectual merit: Global environmental change will affect crop production and plant ecosystems, and inevitably impact the overall health of the human population. In order to deal with changes in crop production caused by global climate change, tt is crucial to understand how plants sense environmental signals. It is known that various external signals trigger elevations in cytosolic Ca2+ concentration ([Ca2+]i), which are mediated by Ca2+ channels in the plasma membrane and/or endomembranes. However, the molecular nature of these Ca2+ channels remains largely elusive in plants. The long-term goal of the PI is to elucidate the Ca2+ machinery in plants. The PI and his research group have used an expression cloning approach and isolated a gene encoding NON-SELECTIVE CALCIUM CHANNEL 1 (NSC1) in Arabidopsis. NSC1 is localized likely to the nuclear membrane in planta, forms Ca2+-permeable channels and mediates Ca2+ flux. Interestingly, NSC1 regulates basal [Ca2+]i and B. subtilis biofilm formation in roots. The specific aims of the project are to (a) characterize the ion channel properties of NSC1 using heterologous expression systems, (b) reconstitute the NSC1 into planar lipid bilayers, (c) determine the subcellular localization of NSC1 in Arabidopsis, and (d) determine the roles of NSC1 in controlling basal [Ca2+]i and regulating B. subtilis biofilm formation. As NSC1 is the first endomembrane Ca2+ channel cloned so far that controls [Ca2+]i, this study should allow a better understanding of plant Ca2+ machinery and plant response to the environment.Broader impacts: Acid rain affects tree growth in forests across the world. It depletes Ca2+ from forests and soils, affects Ca2+ homeostasis in trees, and alters their responses to environmental signals, possibly including communication with beneficial microorganisms. The PI is leading a group to study how acid rain affects forests (in China), aiming to identify molecular targets for genetically engineering forest species. This project will provide unique opportunities for professors and students from primarily teaching universities to conduct research. One postdoc, one graduate student and several undergraduate students will participate in this project. Over half of them are female and one is minority. The results from the project will be incorporated into the PI?s three undergraduate courses: Molecular Plant Physiology, Sensory Signal Transduction, and Cell and Developmental Biology. Undergraduate students, including members of underserved minorities, will participate in the research program, as well as professors from primarily undergraduate-serving institutions.

该奖项由2009年美国复苏和再投资法案（公法111-5）资助。主要研究者姓名：裴振明项目编号IOS-0848263项目名称：拟南芥NSC 1 Ca 2+渗透通道的功能分析知识价值：全球环境变化将影响作物产量和植物生态系统，不可避免地影响人类的整体健康。为了应对全球气候变化引起的作物产量变化，理解植物如何感知环境信号至关重要。已知各种外部信号触发细胞溶质Ca 2+浓度（[Ca 2 +]i）的升高，其由质膜和/或内膜中的Ca 2+通道介导。然而，这些Ca 2+通道的分子本质在植物中仍然很难理解。PI的长期目标是阐明植物中的Ca 2+机制。PI和他的研究小组使用表达克隆方法，在拟南芥中分离了编码非选择性钙通道1（NSC 1）的基因。NSC 1可能定位于植物的核膜上，形成Ca ~（2+）渗透通道并介导Ca ~（2+）的流动。有趣的是，NSC 1调节基础[Ca 2 +]i和B。枯草芽孢杆菌生物膜的形成。本项目的具体目标是（a）使用异源表达系统表征NSC 1的离子通道特性，（B）将NSC 1重组为平面脂质双层，（c）确定NSC 1在拟南芥中的亚细胞定位，以及（d）确定NSC 1在控制基础[Ca 2 +]i和调节B中的作用。枯草杆菌生物膜形成。由于NSC 1是迄今为止克隆的第一个控制[Ca 2 +]i的内膜Ca 2+通道，这项研究应该可以更好地了解植物Ca 2+机制和植物对环境的响应。它消耗森林和土壤中的Ca 2+，影响树木中的Ca 2+稳态，并改变它们对环境信号的反应，可能包括与有益微生物的交流。PI正在领导一个小组研究酸雨如何影响森林（在中国），旨在确定基因工程森林物种的分子目标。该项目将为主要教学大学的教授和学生提供进行研究的独特机会。一名博士后，一名研究生和几名本科生将参与这个项目。其中一半以上是女性，一人是少数民族。项目结果将纳入PI？本系有三门本科课程：分子植物生理学、感觉信号转导、细胞与发育生物学。本科生，包括服务不足的少数民族成员，将参加研究计划，以及来自主要本科服务机构的教授。