MRI: Acquisition of a Flow Cytometer Instrument for Undergraduate Research

MRI：为本科生研究购买流式细胞仪

• 批准号: 0921524
• 负责人: Jay Brewster
• 金额: $ 10.86万
• 依托单位: Pepperdine University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2012-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0921524&HistoricalAwards=false
• 关键词: MRI; Acquisition; Flow; Cytometer; Instrument

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).Pepperdine University has acquired a Cell Lab Quanta SC Flow Cytometer system. The flow cytometer instrument will be utilized in our summer undergraduate research programs and Honor's research program in original, student generated research projects. This instrument will be utilized in a variety of undergraduate research projects carried out in the context of the biology department's current summer program funded in part through a NSF-REU site grant. Acquisition of a flow cytometer will allow us to introduce new concepts into the laboratories of our core cell biology, biochemistry and molecular biology courses, several of our upper-division biology elective courses, and related courses in sports medicine and psychology. Each faculty member associated with this grant is tasked to develop a new undergraduate laboratory teaching module that explores an application of flow cytometry in cell structure or function. The grant will provide undergraduate students with access to the latest equipment for research in molecular and cellular biology. Funds will foster undergraduate research in cell and molecular biology to facilitate interest in graduate studies in cell and molecular biology and also provide training to students interested in employment in biotechnology industries. Specific research programs that will be aided through acquisition of this instrument are: (1) detection of stress signal molecules in pre- and post-apoptotic cells; (2) examining downstream targets in Notch signaling of a mixed population of 7F2 cells; (3) examining changes in levels of phosphorylated and total CaMKII in the hippocampus following neural injury; (4) examining cellular signaling in mammalian vascular smooth muscle under normal and short term exercise training regimes; and (5) examining ploidy and hybridization in chaparral plants. In addition, we will be able to connect undergraduate research and teaching in biology with research and teaching conducted in psychology and sports medicine, thus broadening the interest base and student interconnections between these disciplines within Seaver College at Pepperdine University. Results of the research efforts accomplished from this grant will be available on each investigator's web pages and in peer reviewed publications.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。佩珀代因大学获得了细胞实验室Quanta SC流式细胞仪系统。流式细胞仪将用于我们的夏季本科生研究项目和荣誉的原创研究项目，学生生成的研究项目。该仪器将用于生物系当前夏季项目的各种本科生研究项目中，该项目部分由NSF-REU网站资助。获得一台流式细胞仪将使我们能够将新的概念引入我们的核心细胞生物学，生物化学和分子生物学课程，我们的一些高年级生物选修课程以及运动医学和心理学的相关课程。每位与本基金相关的教师的任务是开发一个新的本科实验教学模块，探索流式细胞术在细胞结构或功能中的应用。这项资助将为本科生提供最新的分子和细胞生物学研究设备。基金将促进细胞和分子生物学的本科生研究，以促进对细胞和分子生物学的研究生学习的兴趣，并为有兴趣在生物技术行业就业的学生提供培训。具体的研究项目将通过该仪器的获取得到帮助：(1)检测凋亡前和凋亡后细胞中的应激信号分子；(2)研究7F2混合细胞群Notch信号的下游靶点；(3)检测神经损伤后海马中磷酸化和总CaMKII水平的变化；(4)研究哺乳动物血管平滑肌在正常和短期运动训练下的细胞信号传导；(5)刺槐属植物的倍性和杂交。此外，我们将能够将生物学的本科生研究和教学与心理学和运动医学的研究和教学联系起来，从而扩大佩珀代因大学西弗学院这些学科之间的兴趣基础和学生联系。这项资助所取得的研究成果将在每位研究者的网页和同行评议的出版物上公布。