MRI: Acquisition of a versatile single cell labeling and high resolution multi-channel imaging system.

MRI：获得多功能单细胞标记和高分辨率多通道成像系统。

• 批准号: 0922789
• 负责人: Mark Martindale
• 金额: $ 47.08万
• 依托单位: University of Hawaii
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-01 至 2013-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0922789&HistoricalAwards=false
• 关键词: MRI; Acquisition; versatile; single; cell

The Kewalo Marine Laboratory of the University of Hawaii has acquired a suite of instrumentation for gene delivery and microscopy with support from the National Science Foundation Major Research Instrumentation (MRI) program. Because of their proximal location to marine fauna, marine laboratories provide unprecedented access to a diverse array of research opportunities unavailable at most university campuses. Researchers at the Kewalo Marine Laboratory, uniquely situated adjacent to living coral reefs near downtown Honolulu, use cellular and molecular approaches to investigate a wide variety of biological phenomena utilizing a full range of marine organisms. Research at the Kewalo Marine Lab runs the gamut of studies that includes the evolution of body plan diversity, life history evolution, regeneration, symbiosis, and effects of biofilms and environmental stressors on developing embryos and larvae. This grant funds an integrated system for labeling, tracking, and documenting living single cells over time within the intact organism. Cell labeling strategies include a precision intracellular microinjection apparatus and a gold particle ballistic delivery system, both of which have advantages in particular preparations. Imaging of labeled cells will be performed by multi-channel confocal microscopy that can image the variety of fluorescent proteins/reagents simultaneously required for each preparation while eliminating background noise. The integrated system is effective and adaptable to the wide array of animal models currently utilized at the Kewalo Marine Lab. This system of cell labeling and documentation will allow students/trainees to exploit the most modern techniques to address fundamental problems in cellular, developmental, evolutionary, and environmental biology. Results from the studies will be disseminated by student and faculty presentations at local and national meetings, and in peer-reviewed journals.

夏威夷大学凯瓦洛海洋实验室在国家科学基金会主要研究仪器(MRI)计划的支持下，获得了一套用于基因传递和显微镜检查的仪器。由于靠近海洋动物的地理位置，海洋实验室提供了前所未有的机会，获得了大多数大学校园所没有的各种研究机会。Kewalo海洋实验室位于檀香山市中心附近的活珊瑚礁附近，该实验室的研究人员使用细胞和分子方法，利用各种海洋生物来研究各种生物现象。凯瓦洛海洋实验室的研究开展了一系列研究，包括身体规划多样性的演变、生活史进化、再生、共生，以及生物膜和环境应激源对发育中的胚胎和幼虫的影响。这笔赠款资助了一个集成的系统，用于标记、跟踪和记录完整有机体内随时间推移的活的单细胞。细胞标记策略包括精确的细胞内微量注射装置和金粒子弹道递送系统，这两种方法在特定的制备方法中都有优势。标记细胞的成像将由多通道共聚焦显微镜进行，该显微镜可以同时成像每种制剂所需的各种荧光蛋白质/试剂，同时消除背景噪音。该集成系统是有效的，适用于凯瓦洛海洋实验室目前使用的各种动物模型。这种细胞标记和记录系统将允许学生/实习生利用最现代的技术来解决细胞、发育、进化和环境生物学中的基本问题。研究结果将通过学生和教职员工在地方和国家会议上的陈述以及同行评议的期刊上发布。