MRI: Acquisition of a versatile single cell labeling and high resolution multi-channel imaging system.

在美国国家科学基金会主要研究仪器（MRI）项目的支持下，夏威夷大学Kewalo海洋实验室获得了一套用于基因传递和显微镜的仪器。由于海洋实验室靠近海洋动物，因此提供了前所未有的各种各样的研究机会，而这些机会在大多数大学校园中是无法获得的。Kewalo海洋实验室位于檀香山市中心附近的活珊瑚礁附近，研究人员利用细胞和分子方法研究各种各样的生物现象，利用各种海洋生物。Kewalo海洋实验室的研究范围包括身体结构多样性的进化、生命史进化、再生、共生以及生物膜和环境压力对胚胎和幼虫发育的影响。这项拨款资助了一个综合系统，用于标记、跟踪和记录完整生物体内随时间变化的活单细胞。细胞标记策略包括精密细胞内显微注射装置和金颗粒弹道传递系统，两者在特定制剂中都具有优势。标记细胞的成像将通过多通道共聚焦显微镜进行，该显微镜可以同时成像每种制备所需的各种荧光蛋白/试剂，同时消除背景噪声。该综合系统是有效的，适用于目前在Kewalo海洋实验室使用的各种动物模型。这个系统的细胞标记和文件将允许学生/学员利用最现代的技术来解决细胞，发育，进化和环境生物学的基本问题。研究结果将通过学生和教师在地方和国家会议上的报告以及在同行评议的期刊上传播。

The Kewalo Marine Laboratory of the University of Hawaii has acquired a suite of instrumentation for gene delivery and microscopy with support from the National Science Foundation Major Research Instrumentation (MRI) program. Because of their proximal location to marine fauna, marine laboratories provide unprecedented access to a diverse array of research opportunities unavailable at most university campuses. Researchers at the Kewalo Marine Laboratory, uniquely situated adjacent to living coral reefs near downtown Honolulu, use cellular and molecular approaches to investigate a wide variety of biological phenomena utilizing a full range of marine organisms. Research at the Kewalo Marine Lab runs the gamut of studies that includes the evolution of body plan diversity, life history evolution, regeneration, symbiosis, and effects of biofilms and environmental stressors on developing embryos and larvae. This grant funds an integrated system for labeling, tracking, and documenting living single cells over time within the intact organism. Cell labeling strategies include a precision intracellular microinjection apparatus and a gold particle ballistic delivery system, both of which have advantages in particular preparations. Imaging of labeled cells will be performed by multi-channel confocal microscopy that can image the variety of fluorescent proteins/reagents simultaneously required for each preparation while eliminating background noise. The integrated system is effective and adaptable to the wide array of animal models currently utilized at the Kewalo Marine Lab. This system of cell labeling and documentation will allow students/trainees to exploit the most modern techniques to address fundamental problems in cellular, developmental, evolutionary, and environmental biology. Results from the studies will be disseminated by student and faculty presentations at local and national meetings, and in peer-reviewed journals.