MRI: Acquisition of a Genome Sequencer FLX for High Throughput Sequencing and Genotyping in Genomics Research and Student Training

MRI：获取基因组测序仪 FLX，用于基因组学研究和学生培训中的高通量测序和基因分型

• 批准号: 0923470
• 负责人: Raymond Jones
• 金额: $ 52.66万
• 依托单位: University of Texas at Arlington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-15 至 2012-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0923470&HistoricalAwards=false
• 关键词: MRI; Acquisition; Genome; Sequencer; FLX

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).This Major Research Instrumentation (MRI)award funds the acquisition of a Genome Sequencer FLX System from 454 Life Sciences and Roche Applied Science to help meet the needs of an expanding group of genomics researchers at University of Texas Arlington and the surrounding vicinity. Genetic and genome analyses have achieved a central role in modern biological research and are widely regarded as the key area for scientific discovery in the 21st century. UTA is making major advances in this field, through both strategic hires and investment in infrastructure. Specifically, UTA has renovated a 10,000+ sq.ft space in the Department of Biology to house the Genome Biology Group (GBG) Core Facility, hired 12 genomics faculty over the last five years, and considerable investment in major research equipment. The 454 sequencer, now part of the facility infrastructure, can produce 400,000 DNA sequences of 200-400 bases (equivalent to ~100 million bases of total sequence) in a single 6-8 hour run to support interdisciplinary and inter-institutional collaborative efforts spanning UTA's Department of Biology, Department of Computer Science and Engineering, and School of Nursing. Specific areas of research interest include: environmental transcriptomics, transcriptional profiling of developing systems (e.g., xenopus oocyte models), coral biology, entomology, plant stress physiology, and many others. Results from the research projects will be disseminated through student and faculty presentations at regional and national meetings, and through publication of peer-reviewed journal articles.

该奖项是根据2009年美国复苏和再投资法案（公法111-5）资助的。该重大研究仪器（MRI）奖资助从454生命科学和罗氏应用科学公司收购基因组测序仪FLX系统，以帮助满足德克萨斯大学阿灵顿及周边地区不断扩大的基因组学研究人员的需求。 遗传和基因组分析在现代生物学研究中发挥了核心作用，并被广泛认为是21世纪科学发现的关键领域。UTA通过战略招聘和基础设施投资，在这一领域取得了重大进展。具体来说，UTA已经在生物系翻新了10，000多平方英尺的空间，以容纳基因组生物学组（GBG）核心设施，在过去五年中聘请了12名基因组学教师，并在主要研究设备上进行了大量投资。 454测序仪，现在的设施基础设施的一部分，可以产生200-400个碱基的400，000个DNA序列（相当于总序列的约1亿个碱基）在一个单一的6-8小时运行，以支持跨学科和跨机构的合作努力跨越UTA的生物系，计算机科学与工程系，和护理学院。 研究兴趣的具体领域包括：环境转录组学，发展中系统的转录谱（例如，非洲爪蟾卵母细胞模型）、珊瑚生物学、昆虫学、植物应激生理学以及许多其他领域。 研究项目的结果将通过学生和教师在区域和国家会议上的演讲以及通过出版同行评审的期刊文章进行传播。