Comparative analysis of miRNA networks regulating flowering

调控开花的miRNA网络的比较分析

• 批准号: 196931075
• 负责人: Professor Dr. Detlef Weigel
• 金额: --
• 依托单位: Abteilung Molecular Biology
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/196931075?language=en
• 关键词: Comparative; analysis; miRNA; networks; regulating

In the past 15 years we have learned a great deal about the genetic and molecular basis of flowering time control, particularly in two species, the dicot Arabidopsis thaliana and the monocot rice. Transferring this knowledge to other crops and trees has, however, not always been straight-forward, because of two main reasons: First, we have an insufficient understanding of conservation and divergence of flowering time networks within and between species. Second, the pleiotropic effects of flowering time regulators are only poorly understood. Here, we propose a comparative analysis in the Brassicaceae, which include both the model plant A. thaliana and Brassica crops, to investigate two microRNA (miRNA) modules controlling flowering as well as other aspects of plant development and physiology: miR156 and its SPL transcription factor targets, and miR172 and its AP2-related targets. Our integrated program incorporates (i) sequence-driven approaches that exploit the availability of a rapidly increasing number of genome sequences in the Brassicaceae, coupled with ultra-high throughput sequencing of small RNA populations; (ii) comparative phenotypic analyses in several species; and (iii) genetic screens that make use of the incredible ease with which novel mutations can be mapped and molecularly identified due to recent advances in next-generation sequencing technologies.

在过去的15年里，我们已经了解了大量的开花时间控制的遗传和分子基础，特别是在两个物种，双子叶植物拟南芥和单子叶植物水稻。然而，将这一知识转移到其他作物和树木并不总是直截了当的，这主要是因为两个原因：首先，我们对物种内部和物种之间开花时间网络的保护和分歧了解不够。其次，开花时间调节剂的多效性作用知之甚少。在这里，我们提出了一个比较分析，在菊科，其中包括模式植物A。拟南芥和芸苔属作物，研究控制开花以及植物发育和生理学的其他方面的两个microRNA（miRNA）模块：miR 156及其SPL转录因子靶标，以及miR 172及其AP 2相关靶标。我们的综合计划包括：（i）序列驱动的方法，利用了快速增加的拟南芥科基因组序列的可用性，再加上小RNA群体的超高通量测序;（ii）几个物种的比较表型分析;以及（iii）基因筛选，利用新的突变可以映射和分子鉴定的令人难以置信的容易，由于最近的进展，代测序技术。