Chemoenzymatic methods for automated carbohydrate synthesis

化学系的化学合成(SYN)计划支持加州大学戴维斯分校的陈曦教授开发高效的化学酶方法，这种方法可以很容易地适用于复杂碳水化合物的自动合成。多步一锅多酶工艺被设计用于从简单的单糖或双糖合成标记的复杂碳水化合物。该方法允许高效的液相合成，并辅之以简易的柱纯化。将建议的方法应用于碳水化合物的自动合成，将使非专业人员也能轻松获得主要的天然哺乳动物碳水化合物。建议的研究涉及有机化学、生物化学和分子生物学领域的多学科前沿研究，为研究生、本科生和暑期高中生提供了极好的培训机会。这里提出的新方法将使复杂碳水化合物的有效生产成为可能，而复杂碳水化合物是生物系统中的重要分子。所获得的产品将使人们更好地了解它们的功能。

The Chemical Synthesis (SYN) Program in the Division of Chemistry supports the work of Professor Xi Chen of the University of California-Davis on developing efficient chemoenzymatic methods which can be readily adapted for automated synthesis of complex carbohydrates. Multistep one-pot-multienzyme processes are designed to synthesize tagged complex carbohydrates from simple mono- or disaccharides. The method allows efficient solution-phase synthesis complemented with facile column purification. The adaption of the proposed method to automated synthesis of carbohydrate will allow easy access to major naturally occurring mammalian carbohydrates even by non-specialists.The proposed research involves multi-disciplinary cutting-edge research in the fields of organic chemistry, biochemistry, and molecular biology, providing excellent training opportunity for graduate students, undergraduate students, and summer high school students. The new method proposed here will allow the efficient product of complex carbohydrates which are important molecules in biological systems. The products obtained will lead to a better understanding of their functions.