A high-throughput analysis of the protein coding genes that direct shell formation in the mollusc Lymnaea stagnalis.

对指导软体动物 Lymnaea stagnalis 壳形成的蛋白质编码基因进行高通量分析。

• 批准号: 200068432
• 负责人: Professor Dr. Daniel Jackson
• 金额: --
• 依托单位: Abteilung Geobiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/200068432?language=en
• 关键词: throughput; analysis; protein; coding; genes

Little is known about the genetic and molecular mechanisms by which animals make hard body parts, also known as biominerals. To date, tens of proteins have been functionally demonstrated to play roles in bone, shell and skeleton formation. This is in contrast to the hundreds of gene products that are thought to be involved in these processes. By using high throughput methods in combination with next generation sequence datasets already in hand, we will isolate and characterise the majority of the gene products involved in shell formation in the freshwater pond snail Lymnaea stagnalis. This high throughput dataset will be the first of its kind for any mollusc, a group of animals whose evolutionary success is largely due to a wide variety of shell morphologies. These results will provide deep insight into the processes that supported the early evolution of multicellular animal life. According to the fossil record, soft-bodied multicellular animals were rapidly replaced by animals with hard endo- and exo-skeletons during a period over 540 million years ago known as the 'Cambrian explosion'. The evolution of an ability to biomineralise is therefore thought to have been one factor that supported the diversification of complex animal life. A comprehensive catalog of the genes responsible for molluscan shell formation will therefore significantly contribute to our understanding of how the ability to biomineralise evolved.

关于动物制造坚硬身体部位（也称为生物矿物）的遗传和分子机制，人们知之甚少。迄今为止，数十种蛋白质已被证明在骨、壳和骨骼形成中发挥作用。这与被认为参与这些过程的数百种基因产物形成对比。通过使用高通量的方法，结合下一代序列数据集已经在手，我们将分离和测序的大多数基因产物参与壳形成的淡水池塘蜗牛滞流。这个高通量数据集将是任何软体动物的第一个此类数据集，软体动物是一组动物，其进化成功在很大程度上是由于各种各样的外壳形态。这些结果将为支持多细胞动物生命早期进化的过程提供深入的见解。根据化石记录，在5.4亿年前被称为“寒武纪大爆发”的时期，软体多细胞动物迅速被具有坚硬内骨骼和外骨骼的动物取代。因此，生物矿化能力的进化被认为是支持复杂动物生命多样化的一个因素。因此，一个全面的目录的基因负责软体动物壳的形成将显着有助于我们了解如何生物矿化的能力演变。