Acquisition of Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometer (MALDI-TOF)

获取基质辅助激光解吸/电离飞行时间质谱仪 (MALDI-TOF)

• 批准号: 1048655
• 负责人: Sarah Green
• 金额: $ 18万
• 依托单位: Michigan Technological University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-12-15 至 2013-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1048655&HistoricalAwards=false
• 关键词: Acquisition; Matrix; Assisted; Laser; Desorption

With this award from the Chemistry Research Instrumentation and Facilities: Multi-user (CRIF:MU) program, Professor Sarah Green and colleagues Lanrong Bi, Shiyue Fang, Haiying Liu and Martin Thompson from Michigan Technological University will acquire a benchtop matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer. The award will enhance research training and education at all levels, especially in areas of study such as (a) DNA sequencing using solid phase capturable di-deoxynucleotides, (b) oligonucleotide purification and block gene expression, (c) design and synthesis of highly water-soluble fluorescent conjugated glycopolymers and glycodendrimers, (d) dendrimers with multiply-bonded dimetallic cores, and (e) analysis of free and glycoprotein-bound glycan structures.Mass spectrometers (MS) are used to identify the chemical composition of a sample by measuring the mass of the molecular constituents in the sample after they are ionized and detected by the mass spectrometer. Matrix-assisted laser desorption ionization (MALDI) is a technique for preparing the sample to be ionized by a laser before injection into the mass spectrometer. The time of flight (TOF) mass analyzer has high sensitivity and mass accuracy to assist the analyses and interpretation of the resultant data. This open access instrument will be available for training students in this analytical tool. It will be used by undergraduate and graduate students in research and in laboratory instruction courses and in outreach activities. It will also enhance collaborations with research groups outside the department that use chemical methods, including those in Forestry, Biological Sciences, Chemical Engineering and Biomedical Engineering.

With this award from the Chemistry Research Instrumentation and Facilities: Multi-user (CRIF:MU) program, Professor Sarah Green and colleagues Lanrong Bi, Shiyue Fang, Haiying Liu and Martin Thompson from Michigan Technological University will acquire a benchtop matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer.该奖项将在各个层面上增强研究培训和教育，尤其是在研究领域，例如（a）使用可捕获的二氧化核苷酸的DNA测序，（（b）寡核苷酸纯化和基因表达，（C）高度水溶解的含量含量的含量含量的糖粉和胶状糖果（C）二脂核以及（e）分析游离和糖蛋白结合的聚糖结构。质谱仪（MS）用于通过测量样品在样品被电离和检测到质谱仪后测量样品后通过测量样品中分子成分的质量来识别样品的化学成分。基质辅助激光解吸电离（MALDI）是一种技术，可以使样品在注入质谱仪之前通过激光使样品电离。飞行时间（TOF）质量分析仪具有高灵敏度和质量准确性，可以帮助对所得数据的分析和解释。这种开放访问工具将用于此分析工具中的培训学生。本科生和研究生将在研究，实验室教学课程和外展活动中使用它。它还将加强与使用化学方法的部门以外的研究小组的合作，包括林业，生物科学，化学工程和生物医学工程。