Acquisition of Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometer (MALDI-TOF)

凭借化学研究仪器和设施：多用户（CRIF：MU）计划的这一奖项，密歇根理工大学的Sarah绿色教授及其同事蓝荣毕、方世跃、刘海鹰和马丁·汤普森将获得一台台式基质辅助激光解吸/电离飞行时间（MALDI-TOF）质谱仪。该奖项将加强各级的研究培训和教育，特别是在以下研究领域：（a）使用固相可捕获的双脱氧核苷酸进行DNA测序，（B）寡核苷酸纯化和阻断基因表达，（c）设计和合成高水溶性荧光共轭糖共聚物和糖树枝状聚合物，（d）具有多重键合双金属核的树枝状聚合物，和（e）分析游离和糖蛋白结合的聚糖结构。质谱仪（MS）用于通过测量样品中的分子组分在它们被电离并被质谱仪检测之后的质量来鉴定样品的化学组成。基质辅助激光解吸电离（MALDI）是一种用于在注入质谱仪之前制备待通过激光电离的样品的技术。飞行时间（TOF）质量分析仪具有高灵敏度和质量精度，以帮助分析和解释所得数据。这一开放获取工具将可用于培训学生使用这一分析工具。本科生和研究生将在研究、实验室教学课程和外联活动中使用该系统。它还将加强与使用化学方法的部门以外的研究小组的合作，包括林业，生物科学，化学工程和生物医学工程。

With this award from the Chemistry Research Instrumentation and Facilities: Multi-user (CRIF:MU) program, Professor Sarah Green and colleagues Lanrong Bi, Shiyue Fang, Haiying Liu and Martin Thompson from Michigan Technological University will acquire a benchtop matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometer. The award will enhance research training and education at all levels, especially in areas of study such as (a) DNA sequencing using solid phase capturable di-deoxynucleotides, (b) oligonucleotide purification and block gene expression, (c) design and synthesis of highly water-soluble fluorescent conjugated glycopolymers and glycodendrimers, (d) dendrimers with multiply-bonded dimetallic cores, and (e) analysis of free and glycoprotein-bound glycan structures.Mass spectrometers (MS) are used to identify the chemical composition of a sample by measuring the mass of the molecular constituents in the sample after they are ionized and detected by the mass spectrometer. Matrix-assisted laser desorption ionization (MALDI) is a technique for preparing the sample to be ionized by a laser before injection into the mass spectrometer. The time of flight (TOF) mass analyzer has high sensitivity and mass accuracy to assist the analyses and interpretation of the resultant data. This open access instrument will be available for training students in this analytical tool. It will be used by undergraduate and graduate students in research and in laboratory instruction courses and in outreach activities. It will also enhance collaborations with research groups outside the department that use chemical methods, including those in Forestry, Biological Sciences, Chemical Engineering and Biomedical Engineering.