Micro-patterned SPR chips for multiplexed imaging analysis of arrays

加州大学河滨分校的程泉教授得到化学系化学测量和成像项目的支持，开发改进的表面等离子体共振（SPR）传感和成像技术，以获得更好的灵敏度和更广泛的应用。该项目的具体目标是：（1）开发微孔限制的SPR芯片;（2）开发基于波导的SPR芯片，以探测结合事件刺激的构象和结构变化;以及（3）使用前两个目标中开发的技术来研究特定的生物分子相互作用。利用波导SPR技术进行的平行和垂直于表面的测量有望获得与当前SPR技术无法获得的构象和结构变化相关的基本信息。如果成功，拟议的研究将导致SPR的重要进展，以及SPR成像的更高灵敏度，这将促进高通量药物筛选，从而影响制药行业和人类健康。参与该项目的学生将接受波导物理，表面功能化化学，膜蛋白和蛋白质配体结合生物学的多学科培训。研究结果将纳入高级生物分析化学课程。

Professor Quan Cheng of the University of California-Riverside is supported by the Chemical Measurement and Imaging Program in the Division of Chemistry to develop improved surface plasmon resonance (SPR) sensing and imaging techniques for better sensitivity and broader applications. The specific aims of the project are: (1) to develop microwell-confined SPR chips; (2) to develop waveguide-based SPR chips to probe conformational and structural changes stimulated by binding events; and (3) to use the techniques developed in the first two aims to study specific biomolecular interactions. Measurements parallel and perpendicular to the surface with waveguide SPR are expected to yield fundamental information pertinent to conformational and structural changes not obtainable with current SPR technologies.If successful, the proposed research will lead to important advances in SPR, and better sensitivity with SPR imaging, which will advance high-throughput drug screening and hence impact the pharmaceutical industry and human health. Students involved in the project will receive multidisciplinary training in waveguide physics, chemistry of surface functionalization, and biology of membrane proteins and protein-ligand bindings. The results will be incorporated in advanced bioanalytical chemistry courses.