I-Corps: Plasmonic Microarray Chips for Microbial Identification

I-Corps：用于微生物识别的等离激元微阵列芯片

• 批准号: 2037821
• 负责人: Quan Cheng
• 金额: $ 5万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2037821&HistoricalAwards=false
• 关键词: Corps; Plasmonic; Microarray; Chips; Microbial

The broader impact/commercial potential of this I-Corps project is to enhance the development of plasmonic microchips that enable microbial analysis in complex samples. This technology can have broad application in laboratory research, water quality assessment, food safety, and human health screening. The simplified sample preparation is advantageous for time sensitive testing, and the improved performance offers a technical feasibility for identifying microbial strains and bacterial contaminants using inexpensive bench-top mass spectrometric instruments. The method can be further adopted to identify viruses based on their lipid corona. This technology eliminates issues experienced with older, laborious techniques and accelerates the understanding of microorganisms’ impact in real-time.This I-Corps project explores metal-enhanced plasmonic properties on microarrays for bacterial identification. Manipulation of metal layer structures offers fluorescence imaging localization, high throughput analysis, and enhanced Matrix-Assisted Laser Desorption/Ionization (MALDI) - Mass Spectrometer (MS) performance. The technique is based on lipidomic profiling on microchips, where sample location is pinpointed with fluorescent imaging for more accurate mass spectrometry analysis. The microchip is transferable to other technique platforms for identification of a range of components including metabolites and lipids at the single cell level that may lead to swift microbial identification.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该 I-Corps 项目更广泛的影响/商业潜力是加强等离子体微芯片的开发，从而实现复杂样品中的微生物分析。该技术可广泛应用于实验室研究、水质评估、食品安全和人类健康筛查。简化的样品制备有利于时间敏感的测试，并且改进的性能为使用廉价的台式质谱仪器识别微生物菌株和细菌污染物提供了技术可行性。该方法还可用于根据脂质冠来识别病毒。这项技术消除了旧的、费力的技术所遇到的问题，并加速了对微生物影响的实时了解。这个 I-Corps 项目探索了用于细菌识别的微阵列上的金属增强等离子体特性。金属层结构的操纵提供荧光成像定位、高通量分析和增强的基质辅助激光解吸/电离 (MALDI) - 质谱仪 (MS) 性能。该技术基于微芯片上的脂质组学分析，其中通过荧光成像精确定位样品位置，以进行更准确的质谱分析。该微芯片可转移到其他技术平台，用于在单细胞水平上鉴定包括代谢物和脂质在内的一系列成分，从而可能实现快速微生物鉴定。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。