Plasmonic Substrates for High Performance SPR Detection in the Kretschmann Configuration

用于 Kretschmann 配置中高性能 SPR 检测的等离激元基底

• 批准号: 1413449
• 负责人: Quan Cheng
• 金额: $ 42.41万
• 依托单位: University of California-Riverside
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1413449&HistoricalAwards=false
• 关键词: Plasmonic; Substrates; Performance; SPR; Detection

Supported by the Chemical Measurement and Imaging Program in the Division of Chemistry, and the Instrument Development for Biological Research Program in the Division of Biological Infrastructure, Professor Quan Cheng and his research group at the University of California-Riverside are developing new materials for the detection of biomolecules using surface plasmon resonance (SPR) methods. SPR is a type of measurement where the light reflected from a metal surface or scattered from metal particles of nanometer dimensions can provide information about the molecules or chemical reactions occuring at the metal surface. SPR-based biosensing technology provides effective tools for study of molecular interactions, facilitates the understanding of biological chemistry, and related fields such as drug discovery. This research focuses on coupling of propagating surface plasmon polaritons (SPPs) and localized surface plasmons in the substrate using the Kretschmann configuration. Several structures such as aligned nanorods and nanovoids are employed to generate new platforms to improve the coupling and thus the detection performance. Specific goals of the research in this work include: 1) developing plasmonic nanoparticle films and 2-D periodic nanostructures as SPR substrates for enhanced label-free analysis; 2) developing ordered porous films on the Au substrate to induce strong optical/plasmonic coupling that improves SPR detection; 3) applying the newly developed SPR substrates for analysis of molecular interactions that involve membrane proteins and aptasensing. LDI-MS analysis with the substrates will be explored as well to systematically evaluate the scope and pragmatism of on-chip orthogonal detection. The highly interdisciplinary nature of this research program also offers diverse opportunities from which students can gain experience when dealing with different problems in surface chemistry, nanotechniques, optics and bioanalytical chemistry.

在化学系化学测量与成像项目和生物基础设施系生物研究仪器开发项目的支持下，加州大学河滨分校的Cheng教授和他的研究小组正在开发利用表面等离子体共振（SPR）方法检测生物分子的新材料。SPR是一种测量类型，其中从金属表面反射或从纳米尺寸的金属颗粒散射的光可以提供关于在金属表面发生的分子或化学反应的信息。基于SPR的生物传感技术为分子相互作用的研究提供了有效的工具，促进了对生物化学和相关领域（如药物发现）的理解。 本研究的重点是使用Kretschmann配置的传播表面等离子体激元（SPPs）和本地化的表面等离子体激元在衬底中的耦合。几种结构，如对齐的纳米棒和纳米空隙被用来产生新的平台，以提高耦合，从而提高检测性能。本研究的具体目标包括：1）开发等离子体纳米颗粒膜和二维周期性纳米结构作为SPR基底，用于增强无标记分析; 2）在Au基底上开发有序多孔膜，以诱导强的光学/等离子体耦合，从而改善SPR检测; 3）将新开发的SPR基底应用于分析涉及膜蛋白和适体传感的分子相互作用。LDI-MS分析与基板将探索，以及系统地评估芯片上正交检测的范围和实用性。该研究计划的高度跨学科性质也提供了多样化的机会，学生可以从中获得经验，在处理表面化学，纳米技术，光学和生物分析化学的不同问题。