Die genetischen Grundlagen thermischer Akklimatisation: Vom Phänotyp zum Genotyp

热适应的遗传基础：从表型到基因型

• 批准号: 201142849
• 负责人: Professor Dr. Klaus Fischer, Ph.D.
• 金额: --
• 依托单位: Abteilung Biologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2014-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/201142849?language=en
• 关键词: Die; genetischen; Grundlagen; thermischer; Akklimatisation

Despite much recent progress in the general understanding of adaptation at the phenotypic level, our knowledge on the genetic mechanisms underlying adaptation, specifically how the genotype maps onto the adaptive phenotype, is still in its infancy. Amongst others due to anthropogenic global change understanding the mechanisms that underpin adaptive processes is of paramount importance. Against this background I will explore the genetic mechanisms underlying adaptive phenotypic plasticity in the tropical butterfly Bicyclus anynana. In this species phenotypic plasticity plays a crucial role for its adaptation to seasonal environments. Explicitly I will (1) investigate the phenotypic (fitness) effects of environmental manipulations, (2) analyze the whole-genome gene expression to uncover genes that are differentially expressed and thus underpin plastic responses, (3) verify the contribution of candidate genes selected from above through qPCR, and (4) investigate the contribution of selected key candidate genes to phenotypic variation by using natural allelic variation. The combination of techniques ranging from ecology to state-of-the-art molecular approaches and the use of ecologically relevant environmental factors in a species greatly relying on plastic responses are expected to generate important insights into the genetic mechanisms underlying adaptive processes.

尽管最近在表型水平上对适应的一般理解取得了很大进展，但我们对适应的遗传机制的认识，特别是基因型如何映射到适应表型上，仍然处于起步阶段。除其他外，由于人为的全球变化，了解适应过程的机制至关重要。在此背景下，我将探讨适应性表型可塑性的热带蝴蝶Bicyclus anynana的遗传机制。在该物种中，表型可塑性对于其适应季节性环境发挥着至关重要的作用。Explanatory我将（1）调查环境操作的表型（适应性）效应，（2）分析全基因组基因表达以揭示差异表达的基因，从而支持可塑性反应，（3）通过qPCR验证从上面选择的候选基因的贡献，（4）通过使用自然等位基因变异调查选择的关键候选基因对表型变异的贡献。从生态学到最先进的分子方法，以及在一个非常依赖塑料反应的物种中使用生态相关的环境因素的技术相结合，有望对适应过程的遗传机制产生重要的见解。

项目成果

Interactive effects of acclimation temperature and short‐term stress exposure on resistance traits in the butterfly Bicyclus anynana

驯化温度和短期应激暴露对双环蝴蝶抗性特征的交互影响

• DOI: 10.1111/phen.12065
• 发表时间: 2014
• 期刊: Physiological Entomology
• 影响因子: 1.5
• 作者: Isabell Karl;Marlen Becker;Tjorven Hinzke;Melanie Mielke;Maria Schiffler ;Klaus Fischer
• 通讯作者: Klaus Fischer