DISSERTATION RESEARCH: Patterns of Gene Expression in an Allopolyploid Taxon of Recurrent Origin: A Case Study of the Polypod Ferns

论文研究：轮回异源多倍体分类群中的基因表达模式：多足蕨类植物的案例研究

• 批准号: 1110775
• 负责人: Kathleen Pryer
• 金额: $ 1.5万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-06-01 至 2014-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1110775&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Patterns; Gene; Expression

Polyploidy, or whole genome duplication, is an important evolutionary mechanism, especially in plants. Most notably, it can lead to differential expression of the duplicated genes. Gene expression patterns in allopolyploids (polyploids resulting from hybridization between two or more distinct species) are particularly intriguing because they reveal the consequences of uniting divergent genomes within a single nucleus. Many naturally-formed allopolyploids are known to form recurrently, resulting in species composed of multiple, independently derived lineages. Recurrent origins of allopolyploids were first documented in the ferns, where they appear to be especially common. Do these independent origins yield similar gene expression patterns that are governed by ?rules?, or is the process random? This study will use high-throughput genomic sequencing techniques to compare patterns of gene expression within and among independently derived lineages of the allopolyploid fern Polypodium hesperium. This study aims to better understand the effects of gene duplication, hybridization, and recurrent origins on the phenotypic variation and evolutionary potential of polyploid species. Because many crops are polyploids, an improved understanding of the gene expression changes that accompany polyploidization may provide insight into potential crop improvement. The proposed study will also provide a research training opportunity for a motivated undergraduate.

多倍体，或全基因组复制，是一种重要的进化机制，特别是在植物中。最值得注意的是，它可以导致重复基因的差异表达。异源多倍体（由两个或多个不同物种杂交产生的多倍体）中的基因表达模式特别有趣，因为它们揭示了在单个细胞核内联合不同基因组的结果。许多自然形成的异源多倍体是已知的形成递归，导致物种组成的多个，独立衍生的谱系。异源多倍体的循环起源首先在蕨类植物中被记录下来，在那里它们似乎特别常见。这些独立的起源是否产生了相似的基因表达模式？规则？还是随机的本研究将使用高通量基因组测序技术，比较基因表达模式的异源多倍体蕨类植物水龙骨hesperium独立衍生的谱系内和之间。本研究旨在更好地了解基因复制、杂交和轮回起源对多倍体物种表型变异和进化潜力的影响。由于许多作物是多倍体，因此对伴随多倍体化的基因表达变化的更好理解可能为潜在的作物改良提供见解。拟议的研究还将为有动力的本科生提供研究培训机会。