CAREER: Deep-UV resonance Raman spectroscopy for detection of protein insertion into lipid vesicles and determination of transmembrane content

职业：深紫外共振拉曼光谱检测蛋白质插入脂质囊泡并测定跨膜含量

• 批准号: 1151533
• 负责人: Renee Jiji
• 金额: $ 60万
• 依托单位: University of Missouri-Columbia
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-07-01 至 2018-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1151533&HistoricalAwards=false
• 关键词: CAREER; Deep; UV; resonance; Raman

This CAREER award by the Chemical Measurement and Imaging (CMI) program supports work by Professor Renee Jiji at the University of Missouri-Columbia to create an innovative research program focused on enhancing structural analysis of transmembrane (TM) proteins using deep-ultraviolet resonance Raman (UVRR) spectroscopy. This research will establish the unique UVRR spectral features of TM proteins and the degree to which solvent exclusion and vibrational coupling over the membrane embedded structural elements play a role in unique resonance enhancement found in the UVRR spectrum of TM proteins. The methods in this proposal will lay the foundation for future structure and functional studies of TM proteins and make the interpretation of the their UVRR spectra more facile. In sum, this research will enable simultaneous resolution of spectral contributions from both solvent accessible and inaccessible regions of TM proteins and the quantification TM protein content, which currently is untenable by a single technique.The broader impacts of this research include outreach programs to provide high school students with the hands-on experience integral to their becoming adept at using scientific instrumentation, and to generate greater interest in chemistry and chemical analysis at the high school level. An initial needs assessment will give an understanding of high school teachers' perceived current needs and iteratively inform the development of hands-on activities. A spectroscopy mini-unit will be designed and implemented to address the assessed needs. Single-day teacher development workshops featuring spectroscopy will increase the teachers' comfort level with scientific and spectroscopic instrumentation while multi-workshops will expand high school students exposures to research instrumentation, highlight applied areas of chemistry, and provide students with stimulating and in depth activities, thereby fostering a greater understanding and interest in chemistry and its professional applications.

化学测量和成像（CMI）计划的职业奖支持密苏里大学哥伦比亚分校的Renee Jiji教授的工作，以创建一个创新的研究计划，专注于使用深紫外共振拉曼（UVRR）光谱法增强跨膜（TM）蛋白的结构分析。这项研究将建立TM蛋白独特的UVRR光谱特征，以及在TM蛋白UVRR光谱中发现的独特共振增强中，膜嵌入结构元素的溶剂排斥和振动耦合发挥作用的程度。该方法将为TM蛋白的结构和功能研究奠定基础，并使其UVRR光谱的解释更加容易。总之，这项研究将能够同时分辨TM蛋白质溶剂可及和不可及区域的光谱贡献，并定量TM蛋白质含量，这是目前无法通过单一技术实现的。这项研究的更广泛影响包括推广计划，为高中生提供实践经验，使他们能够熟练使用科学仪器，并在高中阶段对化学和化学分析产生更大的兴趣。初步需求评估将了解高中教师的感知当前的需求，并反复通知动手活动的发展。将设计和实施一个光谱学微型单元，以满足评估的需求。以光谱学为特色的为期一天的教师发展研讨会将提高教师对科学和光谱仪器的舒适度，而多个研讨会将扩大高中学生对研究仪器的接触，突出化学的应用领域，并为学生提供刺激和深入的活动，从而促进对化学及其专业应用的更大理解和兴趣。