Synthesis and Studies of Water-Soluble, Alpha-Helical Polypeptide with Charged Side Chains

带电荷侧链的水溶性α螺旋多肽的合成与研究

• 批准号: 1153122
• 负责人: Jianjun Cheng
• 金额: $ 27.6万
• 依托单位: University of Illinois at Urbana-Champaign
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-08-01 至 2014-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1153122&HistoricalAwards=false
• 关键词: Synthesis; Studies; Water; Soluble; Alpha

In this project funded by the Macromolecular, Supramolecular and Nanochemistry Program of the Chemistry Division, Jianjun Cheng of the University of Illinois at Urbana-Champaign will synthesize and study water-soluble, alpha-helical polypeptides with charged side chains. Polypeptides are important materials with broad applications and characteristic secondary structures. Water-soluble polypeptides bearing charged side chains typically adopt random coil conformations due to side-chain charge repulsion. In this study, we aim to develop water-soluble, alpha-helical polypeptides by elongating charge-containing amino-acid chains to position the charges distally from the polypeptide backbone and then to study their biophysical properties. Preliminary studies will be performed to test these polymers for use in cell penetration and bacterial inhibition. The broader impacts involve training undergraduate and graduate students, participating in summer high school outreach and undergraduate broadening participation activities at the University of Illinois, and disseminating research results through publications in journals and presentations at conferences. Peptides are chains of chemically linked amino acids and are the components of proteins. This work will expand the repertoire of chemistry that can be used to create new large molecules that mimic natural biomaterials and will lead to a better understanding of how peptides can be engineered to form stable secondary structures, such as an alpha-helix, in water. The results of these studies could have many important long term impacts on applications in which protein-based materials are important, including long term applications in medicine, pharmaceutical processing, and nanotechnology.

在这个由化学系大分子，超分子和纳米化学项目资助的项目中，伊利诺伊大学厄巴纳-香槟分校的Jianjun Cheng将合成和研究具有带电侧链的水溶性α-螺旋多肽。多肽是一类具有广泛用途和独特二级结构的重要物质。 带有带电侧链的水溶性多肽通常由于侧链电荷排斥而采用无规卷曲构象。 在这项研究中，我们的目标是开发水溶性，α-螺旋多肽延长含电荷的氨基酸链的位置远离多肽骨架的电荷，然后研究其生物物理性质。 将进行初步研究，以测试这些聚合物用于细胞渗透和细菌抑制。 更广泛的影响包括培训本科生和研究生，参加夏季高中推广和本科扩大参与活动在伊利诺伊大学，并通过在期刊上发表和在会议上介绍传播研究成果。肽是化学连接的氨基酸链，是蛋白质的组成部分。 这项工作将扩大化学的剧目，可用于创建新的大分子，模仿天然生物材料，并将导致更好地了解如何肽可以工程形成稳定的二级结构，如α-螺旋，在水中。 这些研究的结果可能会对蛋白质材料的重要应用产生许多重要的长期影响，包括在医学，制药加工和纳米技术中的长期应用。