Confocal microscopy at Hopkins Marine Station

斯坦福大学获得了一笔拨款，用于购买一台共聚焦扫描激光显微镜，并在霍普金斯海洋站（HMS）建立一个相关的多用户设备，为加利福尼亚州蒙特利湾半岛的多个海洋实验室提供服务。高分辨率成像现在是细胞和发育生物学过程的当代工具包的重要组成部分。随着蛋白质和核酸荧光标记技术的进步，共聚焦显微镜对许多海洋生物的细胞和组织的三维结构和功能产生了前所未有的深入了解。该技术的一个重要应用是可视化与早期发育、生理学和神经生物学相关的细胞元素和过程。这种技术能力对阐明海洋动物与其环境的相互作用也有更广泛的意义。最终，环境通过对细胞和组织的影响作用于生物体。海洋酸化、脱氧和其他过程将通过对个别生物的影响改变生态系统和渔业。这台显微镜将有助于解决气候变化的各个方面如何影响各种海洋物种的细胞/发育生物学和生理学的关键方面。这台设备将直接使HMS的5个研究实验室受益，并使10名研究生立即受益，并在研究培训学徒期间提供优秀的本科生培训机会。该显微镜将被安置在HMS，但可供当地其他研究机构的研究人员及其学生使用，包括蒙特利湾水族馆，莫斯兰丁的蒙特利湾水族馆研究所和加州州立大学蒙特利湾（CSUMB）。在这些机构中，有许多具有广泛研究兴趣的教师计划在可用时使用这种显微镜。HMS将与CSUMB Bay合作，为麦克奈尔学者项目（McNair Scholars program）提供使用这款显微镜的培训机会，帮助有天赋的少数族裔学生为攻读博士学位做准备。由该设备生成的图像数据将在HMS的定期公开讲座和开放日中突出显示，并在一个致力于海洋无脊椎动物早期胚胎发生和幼虫发育的网络资源上突出显示。HMS和蒙特利湾水族馆（MBA）之间的成像合作将通过MBA广泛建立的外展机制，在正式的公共展览和Facebook帖子中突出图像数据，从而提高公众对海洋无脊椎动物胚胎和幼虫多样性的认识。有关霍普金斯海洋站的更多信息，请访问网站http://www-marine.stanford.edu/。

Stanford University has been awarded a grant to purchase a confocal scanning laser microscope and establish a related multi-user facility at Hopkins Marine Station (HMS) to serve multiple marine laboratories in the Monterey Bay Peninsula, California. High-resolution imaging is now an essential part of the contemporary tool kit of cell and developmental biological processes. Confocal microscopy, along with advances in fluorescent labeling of protein and nucleic acids, has resulted in unprecedented insights into the 3D architecture and function of cells and tissues of many marine organisms. An important application of this technology is visualization of cellular elements and processes related to early development, physiology, and neurobiology. This technical ability also has broader implications for elucidating the interactions of marine animals with their environment. Ultimately, the environment acts on organisms through its effects on cells and tissues. Ocean acidification, deoxygenation and other processes will alter ecosystems and fisheries through effects on individual organisms. This microscope will help address how the various facets of climate change are impacting critical aspects of the cell/developmental biology and physiology of a variety of marine species. This piece of equipment will directly benefit 5 research labs at HMS with an immediate potential benefit to 10 graduate students, and provide outstanding undergraduate training opportunities during research training apprenticeships. The microscope will be housed at HMS but be available to researchers and their students at other research institutions in the local area, including the Monterey Bay Aquarium, Monterey Bay Aquarium Research Institute at Moss Landing and California State University Monterey Bay (CSUMB). There are many faculty at these institutions with a wide range of research interests who plan to use this microscope upon availability. HMS will partner with CSUMB Bay to create training opportunities on this microscope for the McNair Scholars program for gifted minority students to prepare them for doctoral studies. The image data generated from this equipment will be highlighted in the regular public lectures and open houses at HMS, and on a web-based resource dedicated to early embryogenesis and larval development of marine invertebrates. An imaging collaboration between HMS and the Monterey Bay Aquarium (MBA) will develop a greater public awareness of the diversity of embryos and larvae of marine invertebrates by spotlighting image data using the extensive established outreach mechanisms of the MBA both in formal public exhibits and Facebook postings. For more information about Hopkins Marine Station, visit the website at http://www-marine.stanford.edu/.