A novel click chemistry approach to identify learning and memory molecules

一种新颖的点击化学方法来识别学习和记忆分子

• 批准号: DP160103812
• 负责人: Prof Jürgen Götz
• 金额: $ 34.46万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-01-01 至 2019-02-28
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP160103812
• 关键词: novel; click; chemistry; approach; identify

This project seeks to contribute to a deeper understanding at a molecular level of how memory is stored in neurons. Long-term memories do not form immediately after learning. Initially fragile, they become resistant to disruption through a process known as memory consolidation. In a second process, termed reconsolidation, pre-established memories are updated and re-stored. Both processes depend on protein synthesis, but little is known about the particular sets of proteins that are involved. The project plans to apply a novel biochemical protocol to a newly established transgenic mouse model that allows the visualisation and identification of newly synthesised proteins in the hippocampus, a brain area that is critical in memory formation.

该项目旨在帮助在分子水平上更深入地了解记忆如何存储在神经元中。长期记忆不会在学习后立即形成。最初是脆弱的，通过一个被称为记忆巩固的过程，它们变得抵抗破坏。在第二个过程中，称为重新整合，预先建立的记忆被更新和重新存储。这两个过程都依赖于蛋白质的合成，但对所涉及的特定蛋白质组知之甚少。该项目计划将一种新的生物化学方案应用于新建立的转基因小鼠模型，该模型允许可视化和识别海马体中新合成的蛋白质，海马体是记忆形成的关键大脑区域。