Click chemistry to reveal how neurons and glia shape perineuronal nets

点击化学揭示神经元和神经胶质细胞如何塑造神经周围网络

• 批准号: DP230102269
• 负责人: Prof Jürgen Götz
• 金额: $ 32.36万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2023
• 资助国家: 澳大利亚
• 起止时间: 2023-04-01 至 2026-03-31
• 项目状态: 未结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP230102269
• 关键词: Click; chemistry; reveal; how; neurons

The extracellular matrix (ECM) and its perineuronal nets (which are net-like structures with holes wrapped around neurons) are largely underexplored, despite representing a remarkable 20% of the brain’s total volume and having been suggested to be involved in many brain functions. Interestingly, digestion of the ECM improves learning and memory, but deficits return once the ECM has reformed. However, how this ECM remodelling is organised at a cell-type level is not understood. Here we aim to close this knowledge gap, using cutting-edge technology including bioconjugation and ultrasound-mediated cargo delivery. Together, this project aims to contribute to a deeper understanding of this major brain compartment in neuronal function.

细胞外基质(ECM)及其神经周围神经网络(这是网状结构，神经元周围包裹着空洞)尽管占大脑总体积的20%，而且被认为参与了许多大脑功能，但在很大程度上还没有得到充分的探索。有趣的是，ECM的消化改善了学习和记忆，但一旦ECM进行了改革，缺陷就会再次出现。然而，这种ECM重塑是如何在细胞类型的水平上组织的还不清楚。在这里，我们的目标是利用包括生物偶联和超声波介导的货物递送在内的尖端技术来弥合这一知识差距。综上所述，该项目旨在帮助更深入地了解神经元功能中的这一主要脑区。