DISSERTATION RESEARCH: Reticulation and chromosomal evolution in Amaryllidaceae tribe Hippeastreae

论文研究：石蒜科朱顶红的网状结构和染色体进化

• 批准号: 1310839
• 负责人: Pamela Soltis
• 金额: $ 1.9万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-01 至 2015-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1310839&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Reticulation; chromosomal; evolution

This project seeks to examine reticulate evolution in a chromosomally diverse group of Neotropical flowering plants, the Amaryllidaceae (amaryllis family). The generic classification of Amaryllidaceae tribe Hippeastreae, which includes 10-12 genera and ~180 species, including amaryllis, rain lilies, and other ornamentals, remains controversial, despite the horticultural importance of these plants. This group of monocots is putatively descended from an ancient hybridization event, and additional, more recent hybridizations have confounded evolutionary relationships. The main goals are to: 1) assess the extent and nature of reticulate evolution in Hippeastreae; and 2) explore patterns of chromosome evolution in a phylogenetic context. The underlying evolutionary history, or phylogeny, of the group will be investigated with DNA sequences of multiple nuclear genes obtained by next-generation sequencing techniques. Reticulate evolution will be addressed through methods that consider gene tree heterogeneity and network representations of the phylogeny. Chromosomal evolution will be assessed through modeling of chromosome number variation and mapping of cytological characters, including genetic, structural, and numerical differences, onto evolutionary trees. This project will provide general insights into reticulation in plant evolution at different temporal scales and ploidy levels (genome duplication) through an integrative molecular approach.This research will provide the co-PI with significant training opportunities in diverse molecular and cytogenetic techniques, which he will apply as a teacher and researcher in his home country, Chile. Hence, he will contribute to the development of systematics research programs in Chile, where this field currently lacks sufficient expertise. This project will result in a revised classification of Hippeastreae, which will benefit other scientists and horticulturists working with these plants. This classification will be integrated into eMonocot, a global online resource for monocot taxonomy.

该项目旨在研究新热带开花植物——石蒜科（孤挺花科）的染色体多样性群体的网状进化。 石蒜科 Hippeastreae 属包括 10-12 个属和约 180 个物种，包括孤挺花、雨百合和其他观赏植物，尽管这些植物具有园艺重要性，但其属属分类仍然存在争议。 这组单子叶植物被认为是古代杂交事件的后代，而最近的额外杂交已经混淆了进化关系。 主要目标是：1）评估朱顶红网状进化的程度和性质； 2）探索系统发育背景下染色体进化的模式。 该群体的潜在进化历史或系统发育将通过下一代测序技术获得的多个核基因的 DNA 序列进行研究。 网状进化将通过考虑基因树异质性和系统发育的网络表示的方法来解决。 将通过染色体数目变异建模和将细胞学特征（包括遗传、结构和数字差异）映射到进化树上来评估染色体进化。 该项目将通过综合分子方法，对植物进化中不同时间尺度和倍性水平（基因组复制）的网状结构提供一般性见解。这项研究将为共同首席研究员提供多种分子和细胞遗传学技术方面的重要培训机会，他将在他的祖国智利作为教师和研究员应用这些技术。 因此，他将为智利系统学研究项目的发展做出贡献，因为该领域目前缺乏足够的专业知识。 该项目将修订朱顶红的分类，这将有利于其他研究这些植物的科学家和园艺家。 该分类将被整合到 eMonocot 中，eMonocot 是单子叶植物分类学的全球在线资源。