DISSERTATION RESEARCH: The developmental genetics of floral size variation in Saltugilia (Polemoniaceae): Linking cellular phenotypes, genetics, floral size, and pollinator shifts

论文研究：Saltugilia（Polemoniaceae）花大小变异的发育遗传学：将细胞表型、遗传学、花大小和传粉者转变联系起来

• 批准号: 1406650
• 负责人: Pamela Soltis
• 金额: $ 1.93万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2016-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1406650&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; developmental; genetics; floral

The interactions between plants and their pollinators has been gaining increased attention recently due to the recognition of extensive pollinator loss due to anthropogenic activities. One trait that is important for pollinator success is flower size. This project will investigate the cellular and genetic bases of flower size as it relates to selection by pollinators in the phlox family of flowering plants (Polemoniaceae). The goals of this study are: 1) determine the phylogenetic relationships among members of genus Saltugilia, 2) measure cell area of petal epidermal cells in developing flowers using microscopy techniques, 3) identify and isolate the possible genes responsible for the control of flower size based on data from other species, 4) determine the expression profiles of these genes in developing flowers and in species with different pollinators, and 5) demonstrate the function of these genes in regulating floral size.This research will provide the co-PI with training in current genetic and cellular techniques, in addition to training two undergraduates in laboratory techniques. Hands-on modules for high school students will be developed to teach common laboratory techniques such as DNA extractions and polymerase chain reaction. High school teachers will be trained in these protocols to increase the implementation of the modules.

由于认识到人类活动导致授粉媒介大量丧失，植物与其授粉媒介之间的相互作用最近受到越来越多的关注。 对于传粉者成功而言，重要的一项性状是花朵大小。 该项目将研究花大小的细胞和遗传基础，因为它与开花植物福禄考家族（Polemoniaceae）中传粉媒介的选择有关。 本研究的目标是：1) 确定 Saltugilia 属成员之间的系统发育关系，2) 使用显微镜技术测量正在发育的花朵中花瓣表皮细胞的细胞面积，3) 根据其他物种的数据识别和分离负责控制花朵大小的可能基因，4) 确定这些基因在正在发育的花朵和具有不同传粉媒介的物种中的表达谱，5) 证明这些基因在调节花朵大小方面的功能。 除了对两名本科生进行实验室技术培训外，研究还将为联合首席研究员提供当前遗传和细胞技术方面的培训。 我们将为高中生开发实践模块，教授常见的实验室技术，例如 DNA 提取和聚合酶链式反应。 高中教师将接受这些协议的培训，以加强这些模块的实施。