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Ca2+ Waves in Systemic Signaling Networks in Plants

植物系统信号网络中的 Ca2 波

基本信息

批准号：
1329723
负责人：
Simon Gilroy
金额：
$ 63.48万
依托单位：
University of Wisconsin-Madison
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2013
资助国家：
美国
起止时间：
2013-09-01 至 2017-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1329723&HistoricalAwards=false
关键词：
Ca2+Waves Systemic Signaling Networks

项目摘要

Intellectual MeritThe immobile nature of the plant lifestyle requires an ability to continuously monitor local conditions and trigger responses to help cope as these conditions change. Stimuli are often highly localized, yet responses must occur throughout the plant body. Thus, the signaling networks within plants must broadcast information not only within the cells directly receiving the stimulus but also over much longer distances. This project seeks to define how the calcium ion plays a role in this long-distance signaling system. Preliminary work has revealed that in response to local stress, such as wounding of the leaf or increasing levels of salt around the root, waves of Ca2+ propagate from the site of stress perception to distant regions of the plant within seconds. This preliminary analysis has also highlighted a potential role for reactive oxygen species (ROS) and an ion channel named TPC1 in this rapidly propagating Ca2+ wave system. The major research goals to be undertaken in this project are therefore to use plants engineered to produce protein-based Ca2+ reporters to monitor and characterize the Ca2+ changes both at the site of stress perception and in the tissues transmitting this signal. Molecular changes dependent on the Ca2+ wave will then be monitored to define the relationship between the Ca2+ wave and other well-defined plant stress response pathways such as those triggered by ROS and the defense hormones jasmonic- and salicylic-acid. The third goal will be to engineer the TPC1 gene to alter the regulation of this ion channel such that it should no longer respond to ROS or Ca2+. The ability of these mutated versions of the channel to support the movement of the Ca2+ wave will then be assessed. Through pursuing these three goals, this research program will help define how this newly discovered Ca2+ wave signaling system plays a role in helping plants adapt to a constantly changing environment.Broader ImpactsThis project will integrate research and education. Research opportunities for undergraduates will be offered through programs such as the Undergraduate Research Scholars Program and recruitment from members of the Posse Foundation that promote the representation of underrepresented groups in science. Opportunities for high school researchers will also be made available. Along with their research training these scholars will be encouraged to present their work at local, national and international meetings. In addition, efforts at outreach to the general public will be made at the local level through events such as the Wisconsin Institutes of Discovery Science Festival, Wisconsin Science Expeditions and Saturday Science at The Wisconsin Institutes of Discovery. A plant biology movie resource, disseminated through outlets such as YouTube, will also be developed. This resource will be directed towards engaging the general public through movies and explanations of plant responses, whilst also providing them with access to the science behind the images.

植物生活方式的不可移动性要求它们有能力持续监测当地条件，并在这些条件发生变化时触发反应，帮助它们应对。刺激往往是高度局部的，但反应必须发生在整个植物体。因此，植物内部的信号网络不仅要在直接接受刺激的细胞内传播信息，还要传播到更远的距离。该项目旨在确定钙离子如何在这种长距离信号系统中发挥作用。初步研究表明，在对局部胁迫的反应中，如叶片受伤或根周围盐含量增加，Ca2+波在几秒钟内从胁迫感知部位传播到植物的远处区域。这一初步分析也强调了活性氧（ROS）和一个名为TPC1的离子通道在这个快速传播的Ca2+波系统中的潜在作用。因此，本项目的主要研究目标是利用经过工程改造的植物来产生基于蛋白质的Ca2+报告蛋白，以监测和表征胁迫感知部位和传递该信号的组织中的Ca2+变化。然后将监测依赖于Ca2+波的分子变化，以确定Ca2+波与其他明确定义的植物胁迫反应途径之间的关系，例如由ROS和防御激素茉莉酸和水杨酸触发的途径。第三个目标将是设计TPC1基因来改变离子通道的调节，使其不再对ROS或Ca2+作出反应。这些突变的通道支持Ca2+波运动的能力将被评估。通过追求这三个目标，本研究计划将有助于定义这种新发现的Ca2+波信号系统如何在帮助植物适应不断变化的环境中发挥作用。更广泛的影响这个项目将把研究和教育结合起来。本科生的研究机会将通过本科生研究学者计划和招募Posse基金会成员等项目提供，以促进科学界未被充分代表的群体的代表性。还将为高中研究人员提供机会。在进行研究培训的同时，将鼓励这些学者在地方、国家和国际会议上介绍他们的工作。此外，还将在地方一级通过诸如威斯康星发现研究所科学节、威斯康星科学考察和威斯康星发现研究所星期六科学等活动，努力与普通公众进行接触。还将开发通过YouTube等渠道传播的植物生物学电影资源。该资源将通过电影和植物反应的解释吸引公众，同时也为他们提供获取图像背后的科学的途径。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Interplay of Plasma Membrane and Vacuolar Ion Channels, Together with BAK1, Elicits Rapid Cytosolic Calcium Elevations in Arabidopsis during Aphid Feeding

DOI：
10.1105/tpc.17.00136
发表时间：
2017-06-01
期刊：
PLANT CELL
影响因子：
11.6
作者：
Vincent, Thomas R.;Avramova, Marieta;Sanders, Dale
通讯作者：
Sanders, Dale

Control of basal jasmonate signalling and defence through modulation of intracellular cation flux capacity

DOI：
10.1111/nph.14754
发表时间：
2017-12-01
期刊：
NEW PHYTOLOGIST
影响因子：
9.4
作者：
Lenglet, Aurore;Jaslan, Dawid;Farmer, Edward E.
通讯作者：
Farmer, Edward E.

Plants eavesdrop on cues produced by snails and induce costly defenses that affect insect herbivores

植物窃听蜗牛发出的信号并引发影响昆虫食草动物的昂贵防御

DOI：
10.1007/s00442-018-4070-1
发表时间：
2018
期刊：
Oecologia
影响因子：
2.7
作者：
Orrock, John L.;Connolly, Brian M.;Choi, Won-Gyu;Guiden, Peter W.;Swanson, Sarah J.;Gilroy, Simon
通讯作者：
Gilroy, Simon

Using GCaMP3 to Study Ca2+ Signaling in Nicotiana Species