Integration of Hypoxic Signaling Networks

缺氧信号网络的整合

• 批准号: 1121380
• 负责人: Simon Gilroy
• 金额: $ 60万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-15 至 2015-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1121380&HistoricalAwards=false
• 关键词: Integration; Hypoxic; Signaling; Networks

Oxygen is needed for the production of sufficient cellular energy to sustain life. Low oxygen availability, or anoxia, therefore has devastating effects on the viability of many organisms, including plants. Reduction in oxygen levels can come from the plant's own consumption of the available oxygen in densely packed tissues, or from the vagaries of the surroundings such as soil compaction, seasonal flooding and even agricultural practices related to cultivation or irrigation. Surviving these conditions requires the plant to sense the lowering of oxygen availability and shift its energy metabolism to systems to cope with the impending energy deficit. Although this response is essential for survival, our current understanding of this sensory system in plants is highly incomplete, especially at the level of the cellular events that sense and signal a fall in oxygen availability. Therefore, the research in this proposal seeks to more fully characterize the early events that trigger, coordinate and regulate plant responses to low oxygen availability using an integrated approach of imaging of low oxygen-related cellular signaling activities, characterization of associated changes in gene expression and analysis of the responses of mutants impaired in these cell and molecular events. In addition to advancing fundamental knowledge of how plants adapt to the environment, the research in this proposal will also be of long-term relevance to agriculture where seasonal flooding, likely exacerbated by climate change, may become a significant factor in future crop productivity. Integrated into this basic research is also an effort to develop a plant biology movie resource aimed at engaging and educating the general public. This effort will intimately involve undergraduate students in the making and presentation of the movies.

需要氧气才能产生足够的细胞能量来维持生命。因此，低氧可获得性或缺氧对包括植物在内的许多生物的生存能力都有毁灭性的影响。氧气水平的降低可能来自植物自身对密集组织中可用氧气的消耗，或者来自变幻莫测的环境，如土壤压实、季节性洪水，甚至与耕作或灌溉相关的农业实践。要在这些条件下生存下来，植物需要感觉到氧气供应的降低，并将其能量代谢转移到系统，以应对即将到来的能量短缺。尽管这种反应对生存是必不可少的，但我们目前对植物这种感觉系统的了解是高度不完整的，特别是在感知和信号氧气供应下降的细胞事件水平上。因此，这项研究试图通过对低氧相关细胞信号活动的成像、基因表达的相关变化以及分析在这些细胞和分子事件中受损的突变体的反应的综合方法，更全面地表征触发、协调和调节植物对低氧可获得性的反应的早期事件。除了促进植物如何适应环境的基础知识外，这项提案中的研究还将对农业具有长期意义，因为季节性洪水可能会因气候变化而加剧，可能成为未来作物生产力的一个重要因素。这项基础研究还包括努力开发一种植物生物学电影资源，旨在吸引和教育普通公众。这一努力将使本科生密切参与电影的制作和呈现。