Multiphoton Confocal Microscope for Plant Cell Biology

用于植物细胞生物学的多光子共焦显微镜

• 批准号: 0301460
• 负责人: Simon Gilroy
• 金额: $ 33.79万
• 依托单位: Pennsylvania State Univ University Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-09-01 至 2006-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0301460&HistoricalAwards=false
• 关键词: Multiphoton; Confocal; Microscope; Plant; Cell

A grant has been awarded to Pennsylvania State University under the direction of Dr. Simon Gilroy for the acquisition of multi-photon confocal microscope to be housed in a multi-user microscopy imaging facility. The spectral resolving detector and multi-photon capabilities of the microscope will provide new imaging possibilities currently unavailable on the Penn State campus. The microscope will be used and managed by 5 principal groups. Research projects that will directly benefit from the use of these new imaging approaches fall into the broad categories of: (1) investigating signal perception by plants and (2) understanding the cellular basis of morphogenesis. Examples of specific projects that will be possible due to access to the proposed microscope include: (1) Hormonal-regulation of subnuclear dynamics in guard cells, (2) Detection of G-protein activation in living plant cells, (3) Regulation of cytoskeletal dynamics, (4) Control of mitotic progression by cytoskeletal motors, (5) Signal transduction in plant gravity and touch perception, (6) Regulation of pollen tube tip growth by phospholipase C, (7) Control of meiosis and flower development, and (8) Regulation of cell morphogenesis by cell wall properties.The provision of a permanent imaging facility at Penn State will greatly enhance the research infrastructure for biological sciences at the University. In addition, the microscope will directly impact on our efforts to provide collaborative and training opportunities for undergraduate, graduate, postdoctoral and faculty researchers in advanced fluorescence microscopy. Thus, the microscope will be used to train a range of undergraduate researchers and all our incoming plant biology program graduate students. We will also maintain our commitment to providing research opportunities for scientists from around the world to both learn confocal, and now multi-photon, microscopy and to pursue their research using these tools through collaborations.

在西蒙·吉尔罗伊博士的指导下，宾夕法尼亚州立大学获得了一笔赠款，用于购买将安装在多用户显微镜成像设施中的多光子共聚焦显微镜。光谱分辨探测器和显微镜的多光子能力将提供宾夕法尼亚州立大学校园目前无法提供的新的成像可能性。显微镜将由5个主要小组使用和管理。将直接受益于这些新成像方法的研究项目分为以下几大类：（1）研究植物的信号感知和（2）了解形态发生的细胞基础。由于可以使用拟议的显微镜，具体项目的例子包括：（1）保卫细胞亚核动力学的激素调节，（2）活植物细胞中G蛋白活化的检测，（3）细胞骨架动力学的调节，（4）细胞骨架马达对有丝分裂进程的控制，（5）植物重力和触觉的信号转导，（6）磷脂酶C对花粉管顶端生长的调节，（7）减数分裂和花发育的控制，（8）细胞壁特性对细胞形态发生的调节。在宾夕法尼亚州立大学提供永久性成像设施将大大加强该大学生物科学的研究基础设施。此外，显微镜将直接影响我们的努力，为本科生，研究生，博士后和教师研究人员在先进的荧光显微镜提供合作和培训机会。因此，显微镜将用于培训一系列本科研究人员和我们所有即将入学的植物生物学课程研究生。我们还将继续致力于为来自世界各地的科学家提供研究机会，让他们学习共聚焦显微镜和多光子显微镜，并通过合作使用这些工具进行研究。