Multiphoton Confocal Microscope for Plant Cell Biology

在Simon Gilroy博士的指导下，宾夕法尼亚州立大学获得了一笔赠款，用于购置多光子共聚焦显微镜，并将其安置在多用户显微镜成像设施中。该显微镜的光谱分辨探测器和多光子能力将提供宾夕法尼亚州立大学目前无法提供的新的成像可能性。显微镜将由5个主要小组使用和管理。直接受益于这些新成像方法的研究项目可分为以下两大类：(1)研究植物的信号感知；(2)了解形态发生的细胞基础。由于使用了所建议的显微镜，具体项目的例子包括：(1)保护细胞亚核动力学的激素调控，(2)活体细胞g蛋白激活的检测，(3)细胞骨架动力学的调控，(4)细胞骨架马达对有丝分裂进程的控制，(5)植物重力和触觉感知的信号转导，(6)磷脂酶C对花粉管尖端生长的调控，(7)减数分裂和花发育的调控，(8)细胞壁特性对细胞形态发生的调控。宾夕法尼亚州立大学提供的永久成像设备将极大地加强该大学生物科学的研究基础设施。此外，显微镜将直接影响我们的努力，为本科生，研究生，博士后和高级荧光显微镜教师研究人员提供合作和培训机会。因此，该显微镜将用于培养一系列的本科研究人员和我们所有即将进入植物生物学项目的研究生。我们还将继续致力于为来自世界各地的科学家提供研究机会，让他们学习共聚焦显微镜，现在是多光子显微镜，并通过合作使用这些工具进行研究。

A grant has been awarded to Pennsylvania State University under the direction of Dr. Simon Gilroy for the acquisition of multi-photon confocal microscope to be housed in a multi-user microscopy imaging facility. The spectral resolving detector and multi-photon capabilities of the microscope will provide new imaging possibilities currently unavailable on the Penn State campus. The microscope will be used and managed by 5 principal groups. Research projects that will directly benefit from the use of these new imaging approaches fall into the broad categories of: (1) investigating signal perception by plants and (2) understanding the cellular basis of morphogenesis. Examples of specific projects that will be possible due to access to the proposed microscope include: (1) Hormonal-regulation of subnuclear dynamics in guard cells, (2) Detection of G-protein activation in living plant cells, (3) Regulation of cytoskeletal dynamics, (4) Control of mitotic progression by cytoskeletal motors, (5) Signal transduction in plant gravity and touch perception, (6) Regulation of pollen tube tip growth by phospholipase C, (7) Control of meiosis and flower development, and (8) Regulation of cell morphogenesis by cell wall properties.The provision of a permanent imaging facility at Penn State will greatly enhance the research infrastructure for biological sciences at the University. In addition, the microscope will directly impact on our efforts to provide collaborative and training opportunities for undergraduate, graduate, postdoctoral and faculty researchers in advanced fluorescence microscopy. Thus, the microscope will be used to train a range of undergraduate researchers and all our incoming plant biology program graduate students. We will also maintain our commitment to providing research opportunities for scientists from around the world to both learn confocal, and now multi-photon, microscopy and to pursue their research using these tools through collaborations.