Collaborative Research: Systemic Signailng Networks in Arabidopsis

合作研究：拟南芥系统信号网络

• 批准号: 2016177
• 负责人: Simon Gilroy
• 金额: $ 46.91万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2016177&HistoricalAwards=false
• 关键词: Collaborative; Research; Systemic; Signailng; Networks

Stresses such as herbivore or pathogen attack can severely limit plant growth and development, for example, by reducing crop production and impacting food security around the World. Plants possess natural sensory systems that allow them to detect these stresses and then mount defenses against them. Defining how these response systems operate is therefore important in both understanding how plants survive and thrive in the natural world and how to effectively capitalize on these innate responses to improve the resilience of agriculture. Recent research has made key advances in characterizing these signaling systems within the plant, revealing that plants deploy an internal biochemical communication system that broadcasts stress information from its site of perception to the rest of the plant body. This information then triggers the production of defenses such as the accumulation of toxic chemicals, priming even the non-attacked parts of the plant for defense. This communication system operates quickly, spreading information throughout the plant over the course of minutes. Despite such a central role in coordinating each plant’s rapid stress responses, the cellular components that trigger this system and then transmit the stress signal throughout the plant remain poorly defined. This project will focus on characterizing the role of two major cellular messengers, the calcium ion and reactive oxygen species, in propagating these rapid stress signals. The investigation will also explore how amino acids released by the plant may act as initial triggers for this response. This work will train graduate students and postdoctoral fellows to help prepare them for their future careers in research and science. The project will also provide public education on plant stress responses and provide training to students in effective science communication.Recent studies have revealed a rapid systemic signaling system in plants mediated through Ca2+- and reactive oxygen species-dependent events. This research program seeks to extend our understanding of these processes to the levels of the channels and tissue architectures that support this signaling network. The Arabidopsis thaliana Glutamate-Like Receptor (GLR) channels and reactive oxygen species-producing NADPH oxidases have been linked to this rapid systemic propagation of Ca2+-based signals. Therefore, this study will focus on defining the role(s) of these proteins in systemic transmission of local wound, pathogen elicitor (flg22) and salt stresses. The specific aims are to: (1) compare the patterns of Ca2+ and ROS signaling that occur during the initiation and propagation of long-distance signals in response to these stimuli and define the spatial and temporal characteristics of the channels and ROS-related enzymes supporting these activities; and (2) explore how glutamate and other amino acid signals are involved in triggering these long-distance signals. These goals will be accomplished using a combination of bioreporter imaging and mutant and molecular analyses. This study will reveal new insight into the molecular machinery underlying rapid plant systemic signaling. The project will help define the spatial and temporal changes in calcium and reactive oxygen species that relay information about stresses throughout the plant. The research will also help define whether information about each stress is likely encoded in specific signaling dynamics.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

例如草食动物或病原体攻击等压力可以严重限制植物的生长和发育，例如，通过减少农作物的生产并影响世界各地的粮食安全。植物具有自然的感觉系统，使他们能够检测到这些应力，然后对其进行防御。因此，定义这些响应系统如何运作对于理解植物如何在自然世界中生存和繁荣以及如何有效地利用这些对这些最新研究的天生反应的资本化，这在植物内表征这些信号系统方面取得了关键的进步，这表明植物部署了内部生物化学通信系统，从而从其感知到植物体的静脉内广播压力信息。然后，这些信息触发了防御能力的产生，例如有毒化学物质的积累，甚至启动了工厂的未攻击部分进行防御。该通信系统快速运行，在几分钟的时间内通过工厂传播信息。尽管在协调每种植物的快速压力反应中具有如此重要的作用，但触发该系统然后在整个植物中传输应力信号的细胞成分仍然很差。该项目将着重于表征两个主要的细胞信使（钙离子和活性氧）在传播这些快速应激信号中的作用。该投资还将探讨该工厂释放的氨基酸如何作为此反应的初始触发器。这项工作将培训研究生和博士后研究员，以帮助他们为未来的研究和科学职业做好准备。该项目还将提供有关植物压力反应的公共教育，并向学生提供有效的科学交流的培训。当时的研究揭示了通过CA2+和活性氧依赖性事件介导的植物中的迅速系统性信号传导系统。该研究计划旨在将我们对这些过程的理解扩展到支持该信号网络的渠道和组织体系结构的水平。拟南芥谷氨酸受体（GLR）通道和产生活性氧的NADPH氧化物与基于Ca2+的信号的这种快速全身传播有关。因此，这项研究将集中于定义这些蛋白质在局部伤口，病原体诱发剂（FLG22）和盐胁迫的全身传播中的作用。具体目的是：（1）比较在启动和响应这些刺激的长距离信号期间发生的Ca2+和ROS信号的模式，并定义了支持这些活性的通道和ROS相关酶的空间和临时特征； （2）探索谷氨酸和其他氨基酸信号如何触发这些长距离信号。这些目标将通过生物培养剂成像以及突变体和分子分析的组合来实现。这项研究将揭示对快速植物全身信号传导基础的分子机械的新见解。该项目将有助于定义钙和活性氧的空间和临时变化，这些氧气和活性氧中会中继有关整个植物中应力的信息。这项研究还将有助于定义有关每个压力的信息是否可能在特定的信号动态中编码。该奖项反映了NSF的法定任务，并使用基金会的知识分子优点和更广泛的影响评估标准，被视为通过评估来获得珍贵的支持。

项目成果

A touchy subject: Ca2+ signaling during leaf movements in Mimosa

敏感话题：含羞草叶子运动过程中的 Ca2 信号传导

• DOI: 10.1016/j.ceca.2023.102695
• 发表时间: 2023
• 期刊: Cell Calcium
• 影响因子: 4
• 作者: Bakshi, Arkadipta;Swanson, Sarah J.;Gilroy, Simon
• 通讯作者: Gilroy, Simon

Analysis of Plant Root Gravitropism

植物根系向地性分析

• DOI: 10.1007/978-1-0716-2297-1_1
• 发表时间: 2022
• 期刊: Methods in molecular biology
• 作者: Barker, Richard J.;Johns, Sarah;Trane, Ralph;Gilroy, Simon
• 通讯作者: Gilroy, Simon

Wide-Field, Real-Time Imaging of Local and Systemic Wound Signals in Arabidopsis

拟南芥局部和全身伤口信号的广域实时成像

• DOI: 10.3791/62114
• 发表时间: 2021
• 期刊: Journal of Visualized Experiments
• 作者: Uemura, Takuya;Wang, Jiaqi;Aratani, Yuri;Gilroy, Simon;Toyota, Masatsugu
• 通讯作者: Toyota, Masatsugu

The fast and the furious: rapid long-range signaling in plants

• DOI: 10.1093/plphys/kiaa098
• 发表时间: 2021-01-07
• 期刊: PLANT PHYSIOLOGY
• 影响因子: 7.4
• 作者: Johns, Sarah;Hagihara, Takuma;Gilroy, Simon
• 通讯作者: Gilroy, Simon

Pavement cells distinguish touch from letting go

• DOI: 10.1038/s41477-023-01418-9
• 发表时间: 2023-05
• 期刊: Nature Plants
• 影响因子: 18
• 作者: Alexander H. Howell;Carsten Völkner;P. McGreevy;K. Jensen;Rainer Waadt;S. Gilroy;Hans-Henning Kunz;W. Peters;M. Knoblauch