MRI: Acquisition of a Flow Sorter Cytometer to Advance Marine Research and Education

PI要求获得资金以购买最先进的细胞分选流式细胞仪。流式细胞术的使用已成为大多数微生物海洋学研究的核心，在“自适应”采样中至关重要，以评估种群组成和功能的变化。所要求的仪器是一个模块化的，多功能的，高速细胞分选机，将使识别，计数和分选细胞的细微差异，在他们的光学签名。该仪器的一个新奇是其无与伦比的手动调节检测器，需要识别和分类光学特征有细微差异的生物体，这是微生物海洋学研究的核心，涉及多样性、生态系统功能、群落组成变化的生物地球化学影响以及海洋微生物群体的演变。更广泛的影响： 拟议中的流式细胞仪设施不仅将大大促进海洋科学研究，而且将提供给工程，医学和人类学的同事。它将用于本科生和研究生海洋科学学生的新课程，并用于年度研讨会，这将增加UCSB人员和其他研究人员之间的合作。PI在通过媒体采访和与COSEE和学校团体的互动提供访问方面有着良好的记录。该设施的主要价值将是创建一个新的本科生和研究生课程：“流式细胞术在现代微生物海洋学中的应用”。

The PIs request funding to acquire a state-of-the-art cell-sorter flow cytometer. The use of flow cytometry has become central to most research in microbial oceanography, and critical in 'adaptive' sampling to assess shifts in population composition and function. The requested instrument is a modular, versatile, highspeed cell sorter that will enable the identification, enumeration and sorting of cells with subtle differences in their optical signatures. A novelty of the instrument is its unparalleled manual adjustment of detectors required to identify and sort organisms with subtle differences in their optical signatures, which is central in microbial oceanographic studies dealing with diversity, ecosystem function, biogeochemical effects of shifts in community composition, and evolution on marine microbial groups. Broader Impacts: The proposed flow cytometry facility will not only significantly further marine science research but will be available to engineering, medical, and anthropological colleagues. It will be used in a new class for undergraduate and graduate marine science students and in yearly workshops that will increase collaboration between UCSB personnel and other researchers. The PI has a good track record of providing access through media interviews and interactions with COSEE and school groups. A major value of the facility will be the creation of a new undergraduate and postgraduate course: 'Flow Cytometry Applications in Modern Microbial Oceanography'.