MRI: Acquisition of a Quadrupole Time-of-Flight Mass Spectrometer for Proteomics and Metabolomics

MRI：购买用于蛋白质组学和代谢组学的四极杆飞行时间质谱仪

• 批准号: 1337760
• 负责人: Kyongbum Lee
• 金额: $ 38.87万
• 依托单位: Tufts University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-10-01 至 2016-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1337760&HistoricalAwards=false
• 关键词: MRI; Acquisition; Quadrupole; Time; Flight

This proposal seeks to acquire a state-of-the-art mass spectrometry (MS) system for a range of metabolomics and proteomics applications. Mass spectrometry has emerged as the technology of choice for workflows seeking to identify, detect, and/or quantify metabolites and other small molecules as well as proteins and peptides in complex biological samples. The instrument specific benefits include: very fast full-scan acquisition speed, large dynamic range, high resolution,superior sensitivity, and mass accuracy. The proposed QTOF system will support a diverse range of research projects for the PIs as well as a number of major users and collaborators: (1) identifying immunomodulatory microbiota metabolites; (2) in vitro disease model; (3) cardiac tissue engineering and regenerative medicine; (4) lipidoids for nucleic acid delivery; (5)biosynthesis of advanced biofuels; (6) non-invasive monitoring of tissue metabolism;and (7)tendon development and regeneration.

该提案旨在获得一个最先进的质谱（MS）系统，用于一系列代谢组学和蛋白质组学应用。质谱已经成为寻求识别、检测和/或定量复杂生物样品中的代谢物和其他小分子以及蛋白质和肽的工作流程的首选技术。仪器的具体优点包括：非常快的全扫描采集速度、大动态范围、高分辨率、上级灵敏度和质量精度。拟议的QTOF系统将支持PI以及一些主要用户和合作者的各种研究项目：（1）鉴定免疫调节微生物群代谢物;（2）体外疾病模型;（3）心脏组织工程和再生医学;（4）用于核酸递送的类胡萝卜素;（5）先进生物燃料的生物合成;（6）组织代谢的非侵入性监测;（7）生物医学;（8）生物医学;（9）生物医学;（10）生物医学;（11）生物医学;（12）生物医学;（13）生物医学;（14）生物医学;（15）生物医学;（16）生物医学;（17）生物医学;（18）生物医学;（19（7）肌腱的发育和再生。