MRI: Acquisition of a Quadrupole Time-of-Flight Mass Spectrometer for Proteomics and Metabolomics

该提案旨在获得最先进的质谱（MS）系统，用于一系列代谢组学和蛋白质组学应用。质谱分析已成为寻求识别、检测和/或量化复杂生物样品中代谢物和其他小分子以及蛋白质和肽的工作流程的首选技术。该仪器的具体优点包括：非常快的全扫描采集速度、大动态范围、高分辨率、卓越的灵敏度和质量精度。拟议的 QTOF 系统将支持 PI 以及许多主要用户和合作者的各种研究项目：（1）识别免疫调节微生物群代谢物； (2)体外疾病模型； （3）心脏组织工程与再生医学； (4) 用于核酸递送的类脂质； (5)先进生物燃料的生物合成； （6）组织代谢的无创监测；（7）肌腱的发育和再生。

This proposal seeks to acquire a state-of-the-art mass spectrometry (MS) system for a range of metabolomics and proteomics applications. Mass spectrometry has emerged as the technology of choice for workflows seeking to identify, detect, and/or quantify metabolites and other small molecules as well as proteins and peptides in complex biological samples. The instrument specific benefits include: very fast full-scan acquisition speed, large dynamic range, high resolution,superior sensitivity, and mass accuracy. The proposed QTOF system will support a diverse range of research projects for the PIs as well as a number of major users and collaborators: (1) identifying immunomodulatory microbiota metabolites; (2) in vitro disease model; (3) cardiac tissue engineering and regenerative medicine; (4) lipidoids for nucleic acid delivery; (5)biosynthesis of advanced biofuels; (6) non-invasive monitoring of tissue metabolism;and (7)tendon development and regeneration.