MRI: Acquisition of a Quadrupole Time-of-Flight Mass Spectrometer for Proteomics and Metabolomics

该提案寻求获得一种最先进的质谱（MS）系统，用于一系列代谢组学和蛋白质组学的应用。质谱法已经成为工作流程中识别、检测和/或量化复杂生物样品中的代谢物和其他小分子以及蛋白质和肽的首选技术。该仪器的具体优点包括：非常快的全扫描采集速度，大动态范围，高分辨率，卓越的灵敏度和质量精度。拟议的QTOF系统将支持pi以及一些主要用户和合作者的各种研究项目：(1)识别免疫调节微生物群代谢物；(2)体外疾病模型；(3)心脏组织工程与再生医学；(4)输送核酸的脂质；(5)先进生物燃料的生物合成；(6)无创组织代谢监测；(7)肌腱发育和再生。

This proposal seeks to acquire a state-of-the-art mass spectrometry (MS) system for a range of metabolomics and proteomics applications. Mass spectrometry has emerged as the technology of choice for workflows seeking to identify, detect, and/or quantify metabolites and other small molecules as well as proteins and peptides in complex biological samples. The instrument specific benefits include: very fast full-scan acquisition speed, large dynamic range, high resolution,superior sensitivity, and mass accuracy. The proposed QTOF system will support a diverse range of research projects for the PIs as well as a number of major users and collaborators: (1) identifying immunomodulatory microbiota metabolites; (2) in vitro disease model; (3) cardiac tissue engineering and regenerative medicine; (4) lipidoids for nucleic acid delivery; (5)biosynthesis of advanced biofuels; (6) non-invasive monitoring of tissue metabolism;and (7)tendon development and regeneration.