RUI: Structural Determinants of Permeation and Nucleation at a Self-Assembled Interface

RUI：自组装界面渗透和成核的结构决定因素

• 批准号: 1609135
• 负责人: Sunghee Lee
• 金额: $ 32.5万
• 依托单位: IONA UNIVERSITY
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2020-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1609135&HistoricalAwards=false
• 关键词: RUI; Structural; Determinants; Permeation; Nucleation

In this research program, with support from the Macromolecular, Supramolecular and Nanochemistry Program of the Division of Chemistry, Professor Sunghee Lee of Iona College and her undergraduate students gain greater fundamental understanding of how living cells maintain their water balance - a property that is essential to life. They use nature-inspired techniques to create tailor-made particles - these particles are cell-sized water droplets which are used as models for natural cellular systems. A large number of undergraduate students are engaged in this research and many of these students publish peer-reviewed manuscripts. Diverse outreach seminars and science symposia involving high school students and teachers are conducted to inspire and encourage the next generation of STEM educators. Professor Lee reaches out to underrepresented groups in particular. In addition to understanding the water balance in living cells, the research could also have industrial applications in the design of reverse osmosis systems used to purify water.This project focuses on understanding how supramolecular aggregates assembled at liquid-liquid interfaces (including surfactant bilayers) mediate the passage of small molecules and direct biomineralization. Systematic studies determine the effect of bilayer-incorporated materials (e.g., nanomaterials) upon the characteristics of water transport through the droplet interface bilayer (DIB). Experimental activities primarily focus on acquisition of water permeability parameters for osmotic flow in conjunction with the determination of electrical properties, and compositional studies via Raman quantification. Experimental strategies provide insight into how bilayered supramolecular assemblies promote the crystallization of important targets (biominerals and proteins), through nucleation studies of biomineral formation. Biomineralization is the process by which living organisms produce minerals that are often used to harden or stiffen existing tissues.

在这项研究计划中，在化学系大分子，超分子和纳米化学计划的支持下，艾奥纳学院的Sunghee Lee教授和她的本科生们对活细胞如何保持水平衡-一种对生命至关重要的性质-有了更深入的了解。他们使用自然启发的技术来创造量身定制的颗粒-这些颗粒是细胞大小的水滴，用作自然细胞系统的模型。大量的本科生从事这项研究，其中许多学生发表同行评议的手稿。开展了涉及高中学生和教师的各种外联研讨会和科学研讨会，以激励和鼓励下一代STEM教育工作者。李教授特别接触代表性不足的群体。除了了解活细胞中的水平衡外，该研究还可以在用于净化水的反渗透系统的设计中获得工业应用。该项目侧重于了解在液-液界面组装的超分子聚集体（包括表面活性剂双层）如何介导小分子的通过和直接生物矿化。系统的研究确定了双层结合材料（例如，纳米材料）对水通过液滴界面双层（DIB）传输的特性的影响。实验活动主要集中在获取渗透流的水渗透性参数，结合电性能的测定，并通过拉曼定量成分的研究。 实验策略提供了深入了解如何双层超分子组装促进重要目标（生物矿物和蛋白质）的结晶，通过成核研究生物矿物的形成。 生物矿化是生物体产生矿物质的过程，这些矿物质通常用于硬化或修复现有的组织。

项目成果

Ibuprofen and the Phosphatidylcholine Bilayer: Membrane Water Permeability in the Presence and Absence of Cholesterol

布洛芬和磷脂酰胆碱双层：存在和不存在胆固醇时的膜水渗透性

• DOI: 10.1021/acs.langmuir.0c03638
• 发表时间: 2021
• 期刊: Langmuir
• 影响因子: 3.9
• 作者: Wood, Megan;Morales, Michael;Miller, Elizabeth;Braziel, Samuel;Giancaspro, Joseph;Scollan, Patrick;Rosario, Juan;Gayapa, Alyssa;Krmic, Michael;Lee, Sunghee
• 通讯作者: Lee, Sunghee