I-Corps: Label-Free detection of Sialic Acid Using Surface Enhanced Raman Scattering Microscopy

I-Corps：使用表面增强拉曼散射显微镜对唾液酸进行无标记检测

• 批准号: 1663633
• 负责人: Miguel Jose Yacaman
• 金额: $ 5万
• 依托单位: University of Texas at San Antonio
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-11-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1663633&HistoricalAwards=false
• 关键词: Corps; Label; Free; detection; Sialic

The broader and commercial impacts of this I-Corps program are to improve the early-stage detection methods of breast cancer which is expected to reduce morbidity, mortality and rates of recurrence. It is also expected to decrease the overall cost of cancer care and lost productivity from breast cancer treatment and impacts. This screening technology is based on surface-enhanced Raman scattering and offers a noninvasive, high sensitive, rapid and cost-effective method of detection and dynamic monitoring of a breast cancer biomarker sialic acid in saliva. The non-invasive nature of our method helps to accommodate cultural and religious sensitivities that decrease compliance with mammography among certain populations. Moreover, portability and affordability give this technique the potential to promote early diagnosis of breast cancer in low-income, rural or remote areas.This I-Corps project develops a surface-enhanced Raman scattering system for cancer screening. This screening technology offers a noninvasive, high sensitive, rapid and cost-effective method of detection and dynamic monitoring of a breast cancer biomarker sialic acid in saliva. Currently available methods of specific detection and quantification of sialic acid (such as chromatographic methods) are complicated, expensive and time consuming, while more robust techniques (such as colorimetric assays) suffer from non-specificity and interference. This technology may present a versatile and age-independent alternative to standard mammography screening, holding promise for the improved management of breast cancer. Furthermore, it has been reported that sialic acid levels increased in the serum of patients with uterus, lung, colon/rectum, stomach and prostate cancer. Therefore, our method can be potentially used to detect not exclusively breast cancer, but also other types of cancer.

该I-Corps计划的更广泛和商业影响是改善乳腺癌的早期检测方法，该方法有望降低发病率，死亡率和复发率。 预计它将降低癌症护理的总体成本，并因乳腺癌治疗和影响而失去生产力。该筛查技术基于表面增强的拉曼散射，并提供了一种无创，高灵敏，快速和成本效益的检测方法和乳腺癌生物标志物唾液酸的动态监测方法。我们方法的无创性有助于适应文化和宗教敏感性，从而降低了某些人群中乳房X线摄影的依从性。此外，可移植性和负担能力使该技术具有促进低收入，农村或偏远地区早期诊断乳腺癌的潜力。该I-Corps项目开发出一种表面增强的拉曼散射系统，用于癌症筛查。 这种筛查技术提供了一种对唾液中乳腺癌生物标志物唾液酸的检测和动态监测的无创，高灵敏，快速和成本效益的方法。当前可用的唾液酸特异性检测和定量方法（例如色谱方法）是复杂，昂贵且耗时的，而更健壮的技术（例如比色测定法）则遭受了非特异性和干扰。这项技术可能会呈现出多功能且与年龄无关的标准乳房X线摄影筛查替代方案，这对改善乳腺癌的治疗有希望。此外，据报道，子宫，肺，结肠/直肠，胃癌和前列腺癌患者的血清中唾液酸水平升高。因此，我们的方法可能被可能用于检测不仅仅是乳腺癌，而是其他类型的癌症。

项目成果

Diagnosis of breast cancer by analysis of sialic acid concentrations in human saliva by surface-enhanced Raman spectroscopy of silver nanoparticles

• DOI: 10.1007/s12274-017-1576-5
• 发表时间: 2017-11-01
• 期刊: NANO RESEARCH
• 影响因子: 9.9
• 作者: Hernandez-Arteaga, Aida;Zermeno Nava, Jose de Jesus;Ricardo Navarro-Contreras, Hugo
• 通讯作者: Ricardo Navarro-Contreras, Hugo