I-Corps: Label-Free detection of Sialic Acid Using Surface Enhanced Raman Scattering Microscopy

I-Corps：使用表面增强拉曼散射显微镜对唾液酸进行无标记检测

• 批准号: 1663633
• 负责人: Miguel Jose Yacaman
• 金额: $ 5万
• 依托单位: University of Texas at San Antonio
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-11-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1663633&HistoricalAwards=false
• 关键词: Corps; Label; Free; detection; Sialic

The broader and commercial impacts of this I-Corps program are to improve the early-stage detection methods of breast cancer which is expected to reduce morbidity, mortality and rates of recurrence. It is also expected to decrease the overall cost of cancer care and lost productivity from breast cancer treatment and impacts. This screening technology is based on surface-enhanced Raman scattering and offers a noninvasive, high sensitive, rapid and cost-effective method of detection and dynamic monitoring of a breast cancer biomarker sialic acid in saliva. The non-invasive nature of our method helps to accommodate cultural and religious sensitivities that decrease compliance with mammography among certain populations. Moreover, portability and affordability give this technique the potential to promote early diagnosis of breast cancer in low-income, rural or remote areas.This I-Corps project develops a surface-enhanced Raman scattering system for cancer screening. This screening technology offers a noninvasive, high sensitive, rapid and cost-effective method of detection and dynamic monitoring of a breast cancer biomarker sialic acid in saliva. Currently available methods of specific detection and quantification of sialic acid (such as chromatographic methods) are complicated, expensive and time consuming, while more robust techniques (such as colorimetric assays) suffer from non-specificity and interference. This technology may present a versatile and age-independent alternative to standard mammography screening, holding promise for the improved management of breast cancer. Furthermore, it has been reported that sialic acid levels increased in the serum of patients with uterus, lung, colon/rectum, stomach and prostate cancer. Therefore, our method can be potentially used to detect not exclusively breast cancer, but also other types of cancer.

这一I-Corps计划的更广泛的商业影响是改善乳腺癌的早期检测方法，预计将降低发病率、死亡率和复发率。 预计它还将降低癌症护理的总体成本以及乳腺癌治疗和影响造成的生产力损失。该筛选技术基于表面增强拉曼散射，并提供了一种非侵入性、高灵敏度、快速且具有成本效益的方法来检测和动态监测唾液中的乳腺癌生物标志物唾液酸。我们的方法的非侵入性有助于适应文化和宗教敏感性，这些敏感性降低了某些人群对乳房X光检查的依从性。此外，便携性和可负担性使这项技术有可能促进低收入、农村或偏远地区乳腺癌的早期诊断，I-Corps项目开发了一种用于癌症筛查的表面增强拉曼散射系统。 这种筛查技术提供了一种非侵入性，高灵敏度，快速和具有成本效益的方法，用于检测和动态监测唾液中的乳腺癌生物标志物唾液酸。目前可用的特异性检测和定量唾液酸的方法（例如色谱法）复杂、昂贵且耗时，而更稳健的技术（例如比色测定）遭受非特异性和干扰。这项技术可能是一种通用的和与年龄无关的替代标准乳房X光检查筛查，有望改善乳腺癌的管理。此外，据报道，患有子宫癌、肺癌、结肠/直肠癌、胃癌和前列腺癌的患者的血清中唾液酸水平增加。因此，我们的方法不仅可以用于检测乳腺癌，还可以用于检测其他类型的癌症。

项目成果

Diagnosis of breast cancer by analysis of sialic acid concentrations in human saliva by surface-enhanced Raman spectroscopy of silver nanoparticles

• DOI: 10.1007/s12274-017-1576-5
• 发表时间: 2017-11-01
• 期刊: NANO RESEARCH
• 影响因子: 9.9
• 作者: Hernandez-Arteaga, Aida;Zermeno Nava, Jose de Jesus;Ricardo Navarro-Contreras, Hugo
• 通讯作者: Ricardo Navarro-Contreras, Hugo