In vivo und in vitro investigations on the effect of endemic porcine intestinal yeast, Kazachstania slooffiae, on gut microbiota and performance of weaning piglets

断奶期是猪生命周期中最关键的时期之一，常引起健康问题和严重的经济损失。因此，肠道微生物群对动物健康至关重要。初步试验结果表明，在仔猪采食固体饲料后，斯氏哈萨克斯坦酵母菌在仔猪肠道中占优势，但其底物谱尚不清楚。这种酵母特别有趣，因为它似乎与乳酸杆菌积极相互作用，与肠杆菌消极相互作用，这提供了保护潜力。本研究的目的是研究克雷伯氏菌能否在断奶仔猪肠道内有目的地定植。slooffiae和这种酵母是否可以影响猪肠道菌群。在为期4周的试验中，采用454测序技术，测定了口服K.斯卢菲耶。该酵母将通过其全基因组和代谢产物分析进行全面表征。进行体外研究以检查酵母发酵不同底物的能力，从而检查形成的产物。

Weaning can be regarded as one of the most critical periods in the life cycle of pigs and is often related with health disorders and high economic losses. The intestinal microbiota is thereby of crucial importance for the animal health. It was shown in preliminary tests the yeast Kazachstania slooffiae dominates the gut of piglets after intake of solid feed, whereas it is not clear yet its substrate spectrum. This yeast is of special interest, because it seems to interact positively with lactobacilli and negatively with enterobacteria, which offers a protective potential. The aim of the project is to examine whether the intestine of weaning piglets can be purposefully colonized with K. slooffiae and whether this yeast can influence porcine intestinal microbiota. In a 4 weeks experiment, health status, zootechnical parameters and the composition of intestinal microbiota, using 454 sequencing technology, are determined after oral application of K. slooffiae. This yeast will be comprehensively characterized by means of its whole genome and metabolites analyses. In vitro studies are performed to examine the ability of the yeast to ferment different substrates and thereby formed products will be examined.