Super resolution imager sensing system using structured illuminated plasmonic spatial interferometers

使用结构化照明等离子体空间干涉仪的超分辨率成像传感系统

• 批准号: 1807463
• 负责人: Qiaoqiang Gan
• 金额: $ 36万
• 依托单位: SUNY at Buffalo
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-06-01 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1807463&HistoricalAwards=false
• 关键词: Super; resolution; imager; sensing; system

Portable biomedical devices hold significant promises for various applications that have the potential to impact the fight against several global health problems. It was estimated that approximately two-thirds of global cell-phones are being used in the developing world (e.g. Africa, Asia). Therefore, sensitive biomedical devices integrated with smart-phones would yield a promising sensing system and introduce great impact on point-of-care diagnostics in developing countries and resource-limited areas. This proposal aims to develop a monochromatic structured illuminated plasmonic spatial interferometer suitable for highly sensitive sensing on digital cameras (e.g. on desktop microscopes and smart phones). The enhanced sensing performance is enabled by the super resolution strategy of structured illumination integrated with the plasmonic nanostructure developed in this project, which overcomes the resolution limit of the digital cameras. When successfully developed, such highly sensitive interferometers will allow a variety of biosensing applications at a much lower cost, with great societal impact on real time and in situ monitoring of global environmental (e.g. water) quality, liquid-food borne illnesses and personal health conditions. This research will be closely integrated with educational programs in the departments of Electrical Engineering and Biomedial Engineering at University at Buffalo. It will significantly impact the Electrical Engineering and Biomedial Engineering curriculum with its emphasis on experiential learning, and will provide an excellent educational opportunity for graduate and undergraduate student training of the next generation of researchers, educators and global leaders. The two PIs will provide excellent opportunities for undergraduate and graduate training in theoretical modeling, nanofabrication, miniaturized system design and optical super resolution image processing. The main goals of this educational and outreach program are to enhance the educational communication and collaboration, improve the participation of graduate and undergraduate students in cutting-edge researches, for outreach to K-12 students by organizing engineering summer campus, and provide opportunities for under-represented groups. While Surface Plasmon Resonance systems are currently used for label-free sensing, they remain inadequate for use in portable systems as the commercial instrumentation is expensive, complex, bulky and inconvenient for the integration with microfluidic platforms. Therefore, there is an urgent need to develop low-cost, compact, and high performance sensor systems for the ever-increasing sensing applications. Nanoplasmonic sensors are attractive miniaturized platforms to potentially meet these requirements. However, because nanoplasmonic sensors are mostly based on broadband wavelength shift interrogation which requires expensive spectrometers, high throughput sensing is still very challenging. This proposal will develop a plasmonic spatial interferometer structure to transfer the broadband wavelength peak/valley shift to the spatial interference pattern shift at a monochromatic wavelength such that the shift can be directly imaged by digital cameras on microscopes and smart-phones without the need of expensive spectrometers. To further enhance the sensing performance of the proposed system, the super resolution structured illumination strategy will be integrated into the sensor design by introducing a nanopatterned reference slit to generate Moire fringes with low spatial frequency information. This strategy enables a resolution much higher than the resolution limits of the optical imager system, thus allowing ultra-small spatial shift (sub-resolution-limit) to be observable even with portable cameras. The proposed system does not require spectrometer or angular tunable prism coupling system, leading to a significant reduction in device cost and instrumental complexity, especially when realized on smart-phone-based microscope systems. The potential of this sensing system will be demonstrated through the super-resolution-resolved peak/valley shift of 50 nm using inexpensive digital imagers, corresponding to an ultra-small sensing resolution approaching the performance of commercial bulky surface plasmon resonance imager systems.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

便携式生物医学设备在各种应用中具有重要的前景，这些应用有可能影响与几个全球健康问题的斗争。据估计，全球大约三分之二的手机是在发展中世界（例如非洲、亚洲）使用的。因此，与智能手机集成的敏感生物医学设备将产生有前途的传感系统，并对发展中国家和资源有限地区的即时诊断产生重大影响。该提案旨在开发单色结构化照明等离子体空间干涉仪，其适用于数码相机（例如，台式显微镜和智能手机）上的高灵敏度感测。增强的传感性能是通过与在这个项目中开发的等离子纳米结构集成的结构化照明的超分辨率策略来实现的，其克服了数码相机的分辨率限制。当成功开发时，这种高灵敏度干涉仪将允许以低得多的成本进行各种生物感测应用，对全球环境（例如水）质量、液体食物传播的疾病和个人健康状况的真实的实时和原位监测具有巨大的社会影响。这项研究将与布法罗大学电气工程和生物医学工程系的教育计划紧密结合。它将对电气工程和生物医学工程课程产生重大影响，重点是体验式学习，并将为下一代研究人员，教育工作者和全球领导者的研究生和本科生培训提供绝佳的教育机会。这两个PI将为理论建模、纳米制造、微型系统设计和光学超分辨率图像处理方面的本科生和研究生培训提供绝佳的机会。这个教育和推广计划的主要目标是加强教育沟通和合作，提高研究生和本科生在前沿研究的参与，通过组织工程暑期校园推广到K-12学生，并为代表性不足的群体提供机会。虽然表面等离子体共振系统目前用于无标记感测，但它们仍然不足以用于便携式系统，因为商业仪器昂贵、复杂、笨重并且不便于与微流体平台集成。因此，迫切需要开发低成本，紧凑，高性能的传感器系统，以满足日益增长的传感应用。纳米等离子体传感器是潜在地满足这些要求的有吸引力的小型化平台。然而，由于纳米等离子体传感器主要基于宽带波长偏移询问，这需要昂贵的光谱仪，因此高通量感测仍然非常具有挑战性。该提案将开发等离子体空间干涉仪结构，以将宽带波长峰/谷偏移转换为单色波长处的空间干涉图案偏移，使得该偏移可以通过显微镜和智能手机上的数码相机直接成像，而不需要昂贵的光谱仪。为了进一步提高所提出的系统的传感性能，超分辨率结构化照明策略将被集成到传感器设计中，通过引入纳米图案化参考狭缝来产生具有低空间频率信息的莫尔条纹。该策略使得分辨率比光学成像仪系统的分辨率极限高得多，从而允许即使用便携式相机也可观察到超小的空间偏移（亚分辨率极限）。所提出的系统不需要光谱仪或角度可调棱镜耦合系统，从而显著降低设备成本和仪器复杂性，特别是在基于智能手机的显微镜系统上实现时。这种传感系统的潜力将通过使用廉价的数字成像仪实现50 nm的超分辨率分辨峰/谷位移来证明，对应于一个超该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的影响审查进行评估来支持的搜索.

项目成果

Large‐Scale Sub‐1‐nm Random Gaps Approaching the Quantum Upper Limit for Quantitative Chemical Sensing

• DOI: 10.1002/adom.202001634
• 发表时间: 2020-10
• 期刊: Advanced Optical Materials
• 影响因子: 9
• 作者: Nan Zhang;Hai-feng Hu;Matthew H. Singer;Kuang-Hui Li;Lyu Zhou;B. Ooi;Qiaoqiang Gan

Plasmonic Interferometer Array Biochip as a New Mobile Medical Device for Cancer Detection.

等离子体干涉仪阵列生物芯片作为一种用于癌症检测的新型移动医疗设备。

• DOI: 10.1109/jstqe.2018.2865418
• 发表时间: 2019
• 期刊: IEEE journal of selected topics in quantum electronics : a publication of the IEEE Lasers and Electro-optics Society
• 作者: Zeng,Xie;Yang,Yunchen;Zhang,Nan;Ji,Dengxin;Gu,Xiaodong;Jornet,Josep;Wu,Yun;Gan,Qiaoqiang
• 通讯作者: Gan,Qiaoqiang

Intensity-modulated nanoplasmonic interferometric sensor for MMP-9 detection.

• DOI: 10.1039/c8lc01391h
• 发表时间: 2019-03
• 期刊: Lab on a chip
• 影响因子: 6.1
• 作者: Yifeng Qian;Xie Zeng;Yongkang Gao;Hang Li;Sushil Kumar;Qiaoqiang Gan;Xuanhong Cheng;F. Bartoli