The endosome at atomic resolution

原子分辨率的内体

• 批准号: DP160101743
• 负责人: Prof Brett Collins
• 金额: $ 30.74万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-01-01 至 2019-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP160101743
• 关键词: endosome; atomic; resolution

The project seeks to improve understanding of intracellular transport. The transport of proteins is essential for controlling the interactions of a cell with its environment, and for regulating a huge number of cell signalling events. The retromer protein complex is a central mediator of intracellular trafficking in organelles called endosomes. It is vital for normal cell homeostasis in all eukaryotic organisms, and is an emerging target for treatment of human neurodegenerative diseases. This project plans to use a combination of cutting-edge X-ray crystallographic and electron microscopy approaches to develop a multi-scale, pseudo-atomic structure of retromer and key regulatory proteins to understand how this multi-component protein machinery is assembled to control intracellular transport.

该项目旨在提高对细胞内转运的理解。蛋白质的转运对于控制细胞与其环境的相互作用以及调节大量细胞信号事件至关重要。逆转录蛋白复合物是称为内体的细胞器中细胞内运输的中心介质。它对所有真核生物中的正常细胞稳态至关重要，并且是治疗人类神经退行性疾病的新兴靶点。该项目计划使用尖端的X射线晶体学和电子显微镜方法相结合，开发retromer和关键调控蛋白的多尺度伪原子结构，以了解这种多组分蛋白质机制如何组装以控制细胞内转运。