权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

EAGER: Optical Molecular Imaging of Opioid Distribution and its Metabolic Effects in the Brain

EAGER：阿片类药物分布及其在大脑中代谢作用的光学分子成像

基本信息

批准号：
1841539
负责人：
Stephen Boppart
金额：
$ 30万
依托单位：
University of Illinois at Urbana-Champaign
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-15 至 2020-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1841539&HistoricalAwards=false
关键词：
EAGER Optical Molecular Imaging Opioid

项目摘要

This project will develop and demonstrate a unique live-tissue imaging platform that can detect the presence of opioids in live brain slices from mice, and image their effects on the cellular metabolism and coupled neural brain activity. This platform will allow the first visualization of how the presence of opioids affects the metabolism of neurons and astrocytes, and the subsequent neural spontaneous depolarization activity. The establishment and demonstration of this imaging platform will enable future comparative studies using morphine (the prototypical opioid), caffeine, and dopamine to elucidate how opioids differ from non-addictive compounds (e.g. caffeine and anesthetics) and prevalent neurotransmitters (e.g. dopamine) to modulate the cellular metabolism of the brain.This project will impact opioid addiction and related neuroscience and impact the broader research of drug development involving different diseases, organs, and preclinical models. The proposed imaging platform will be generally applicable to drug screening and discovery through preclinical imaging when the opioid is replaced by the drug of interest. The results of this project will be shared amongst the scientific/engineering and pharmaceuticalcommunities, and across wide segments of society in outreach activities. The new imaging andvisualization capabilities will inspire K-12 students to think about how technology can be used to benefit scientific investigations. Outreach activities will include demonstrations of this imaging platform to community groups through annual Engineering Open House events, as well as integration of these technological methods in Prof. Boppart's undergraduate Biophotonics and Biomedical Imaging courses.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目将开发和展示一种独特的活组织成像平台，该平台可以检测小鼠活脑切片中阿片类药物的存在，并对其对细胞代谢和神经脑活动的影响进行成像。该平台将允许首次可视化阿片类药物的存在如何影响神经元和星形胶质细胞的代谢，以及随后的神经自发去极化活动。这一成像平台的建立和演示将使未来使用吗啡的比较研究成为可能（原型阿片类药物），咖啡因，和多巴胺来阐明阿片类药物与非成瘾性化合物的区别（例如咖啡因和麻醉剂）和普遍的神经递质（例如多巴胺）调节大脑的细胞代谢。该项目将影响阿片成瘾和相关的神经科学，并影响更广泛的药物研究。涉及不同疾病、器官和临床前模型的发展。当阿片类药物被感兴趣的药物取代时，所提出的成像平台将通常适用于通过临床前成像进行药物筛选和发现。该项目的成果将在科学/工程和制药界以及社会各阶层的外联活动中分享。新的成像和可视化功能将激发K-12学生思考如何利用技术来促进科学研究。推广活动将包括通过每年的工程开放日活动向社区团体展示这一成像平台，以及将这些技术方法整合到Boppart教授的本科生生物光子学和生物医学成像课程中。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

期刊论文数量（8）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Two-photon microscope using a fiber-based approach for supercontinuum generation and light delivery to a small-footprint optical head.

双光子显微镜使用基于光纤的方法来生成超连续谱并将光传输到小型光学头。

DOI：
10.1364/ol.381571
发表时间：
2020
期刊：
Optics letters
影响因子：
3.6
作者：
Zhao,Youbo;Maguluri,Gopi;DanielFerguson,R;Tu,Haohua;Paul,Kush;Boppart,StephenA;Llano,DanielA;Iftimia,Nicusor
通讯作者：
Iftimia,Nicusor

Depixelation and enhancement of fiber bundle images by bundle rotation

DOI：
10.1364/ao.59.000536
发表时间：
2020-01-10
期刊：
APPLIED OPTICS
影响因子：
1.9
作者：
Renteria, Carlos;Suarez, Javier;Boppart, Stephen A.
通讯作者：
Boppart, Stephen A.

Dynamic Tracking Algorithm for Time-Varying Neuronal Network Connectivity using Wide-Field Optical Image Video Sequences

使用广域光学图像视频序列的时变神经网络连接的动态跟踪算法

DOI：
10.1038/s41598-020-59227-5
发表时间：
2020
期刊：
Scientific Reports
影响因子：
4.6
作者：
Renteria, Carlos;Liu, Yuan-Zhi;Chaney, Eric J.;Barkalifa, Ronit;Sengupta, Parijat;Boppart, Stephen A.
通讯作者：
Boppart, Stephen A.

Simultaneous label-free autofluorescence-multiharmonic microscopy and beyond