CAREER: Dynamic phase-sensitive optical coherenence microscopy to quantify activity in retinal cells

职业：动态相敏光学相干显微镜量化视网膜细胞的活动

• 批准号: 1845801
• 负责人: Jeremy Rogers
• 金额: $ 50万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1845801&HistoricalAwards=false
• 关键词: CAREER; Dynamic; phase; sensitive; optical

Stem cell technology has the potential to provide a pathway for reversing sight damage that occurs with age. A crucial element of reversing sight damage is developing techniques to quantitatively assess the structure as well as the activity and function of individual cells within the retina. Without this monitoring capability, treatments would rely on structure alone, which is not comprehensive. The overall goal of this research project is to develop clinical imaging tools that could provide such features before, during, and after drug and gene therapies. The project's educational activities include development of innovative optics curricula and educational outreach events designed for students of all ages. Outreach activities will be also performed with the Wisconsin Institutes for Discovery (WID). The technical approach calls for advancing phase sensitive optical coherence tomography to study cellular motion in three-dimensional cell cultures. The motivation is that this approach will help develop tools for assessing success of transplanted retinal cells.The core advancement proposed here is to determine if dynamic phase-sensitive optical coherence microscopy will enable imaging of cellular function. This will be done by developing a high resolution dynamic phase-sensitive optical coherence microscope, quantifying phagocytosis in retinal pigment epithelium as a model of disease, and characterizing photoreceptor activity in stem cell-derived organoids.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

干细胞技术有可能为逆转随着年龄增长而出现的视力损伤提供途径。逆转视力损伤的一个关键因素是开发定量评估视网膜内单个细胞的结构、活动和功能的技术。如果没有这种监测能力，治疗将仅仅依赖于结构，这是不全面的。这项研究项目的总体目标是开发临床成像工具，在药物和基因治疗之前、期间和之后提供这些特征。该项目的教育活动包括开发创新的光学课程和为所有年龄段的学生设计的教育推广活动。还将与威斯康星探索研究所（WID）开展外展活动。技术方法要求推进相敏光学相干层析成像来研究三维细胞培养中的细胞运动。动机是这种方法将有助于开发评估移植视网膜细胞成功的工具。这里提出的核心进展是确定是否动态相敏光学相干显微镜将使细胞功能成像。这将通过开发高分辨率动态相敏光学相干显微镜来完成，定量视网膜色素上皮中的吞噬作为疾病模型，并表征干细胞衍生类器官中的光感受器活性。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Measuring the spatial distribution of multiply scattered light using a de-scanned image sensor for examining retinal structure contrast

使用去扫描图像传感器测量多重散射光的空间分布以检查视网膜结构对比度

• DOI: 10.1364/oe.408587
• 发表时间: 2021
• 期刊: Optics Express
• 影响因子: 3.8
• 作者: Sajdak, Benjamin S.;Postlewaite, Jack T.;Eliceiri, Kevin W.;Rogers, Jeremy D.
• 通讯作者: Rogers, Jeremy D.

Noise reduction in supercontinuum sources for OCT by single-pulse spectral normalization

通过单脉冲光谱归一化降低 OCT 超连续谱源的噪声

• DOI: 10.1364/ao.393141
• 发表时间: 2020
• 期刊: Applied Optics
• 影响因子: 1.9
• 作者: Niemeier, Ryan C.;Simmons, Zach J.;Rogers, Jeremy D.
• 通讯作者: Rogers, Jeremy D.

Calibration of liquid crystal variable retarders using a common-path interferometer and fit of a closed-form expression for the retardance curve

使用共路干涉仪校准液晶可变延迟器并拟合延迟曲线的封闭式表达式

• DOI: 10.1364/ao.408383
• 发表时间: 2020
• 期刊: Applied Optics
• 影响因子: 1.9
• 作者: Schnoor, Nicholas P.;Niemeier, Ryan C.;Woods, Aaron L.;Rogers, Jeremy D.
• 通讯作者: Rogers, Jeremy D.

Designing a Compact, Low-Cost FRET Microscopy Platform for the Undergraduate Classroom

为本科课堂设计紧凑、低成本的 FRET 显微镜平台

• DOI: 10.35459/tbp.2019.000117
• 发表时间: 2020
• 期刊: The Biophysicist
• 作者: Rupel, John W.;Sdao, Sophia M.;Johnston, Kadina E.;Nethery, Ethan T.;Gabardi, Kaitlyn A.;Ratliff, Benjamin A.;Simmons, Zach J.;Postlewaite, Jack T.;Kita, Angela M.;Rogers, Jeremy D.
• 通讯作者: Rogers, Jeremy D.