MRI: Acquisition of a Flow Cytometer Cell Sorter for Multidisciplinary Studies at Oakland University

MRI：奥克兰大学购买流式细胞仪细胞分选仪进行多学科研究

• 批准号: 1919572
• 负责人: Luis Villa Diaz
• 金额: $ 44.55万
• 依托单位: Oakland University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-15 至 2022-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1919572&HistoricalAwards=false
• 关键词: MRI; Acquisition; Flow; Cytometer; Cell

An award is made to Oakland University to acquire a Fluorescence-Activated Cell Sorting (FACS) machine to support and enhance multidisciplinary research and educational programs at the institution. The FACS machine will allow undergraduate/graduate students and faculty to participate in research and educational activities that will enhance the mission of the Departments of Biological Sciences and Chemistry as well as the Eye Research Institute. Specifically, the FACS machine will allow for hands-on collaborative research in the areas of amphibian biology, cancer biology, chemistry, DNA repair, developmental biology, immunology, molecular biology, plant genomics, and stem cell biology. The instrument will be incorporated into the science and bioengineering curriculums, as part of teaching laboratories and design projects, and will allow for new course development. This instrument will also allow for student and faculty recruitment and provide a sophisticated service technology to the surrounding region.Flow cytometry combined with cell sorting enables characterizing, defining and sorting of different cell types and subpopulations by analyzing the expression of cell-surface and intracellular molecules. Among some specific projects in which this instrument will be used are the determination of basic biological functions and signaling directed by cell surface proteins in stem cells, the role of endothelial cells on anti-apoptotic pathways, the regulation of pre-mRNA processing in plants, the effects of temperature in amphibian immunology, the development of tubular organs, and basic understanding of DNA repair mechanisms. Results from the research using the instrument will be disseminated in peer-reviewed publications, scientific meeting and to the community throughout outreach activities in which faculty of this institution participate.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

奥克兰大学获得了一个奖项，以获得一个荧光激活细胞分选（FACS）机，以支持和加强该机构的多学科研究和教育计划。流式细胞仪将允许本科生/研究生和教师参加研究和教育活动，这将提高生物科学和化学系以及眼科研究所的使命。具体而言，流式细胞仪将允许在两栖动物生物学，癌症生物学，化学，DNA修复，发育生物学，免疫学，分子生物学，植物基因组学和干细胞生物学领域进行动手合作研究。 该仪器将作为教学实验室和设计项目的一部分，纳入科学和生物工程课程，并将允许开发新的课程。该仪器还将为学生和教师招聘提供便利，并为周边地区提供先进的服务技术。流式细胞术与细胞分选相结合，通过分析细胞表面和细胞内分子的表达，可以对不同的细胞类型和亚群进行表征、定义和分选。 在一些具体的项目中，该仪器将被用于确定基本的生物学功能和干细胞中细胞表面蛋白的信号传导，内皮细胞在抗凋亡途径中的作用，前mRNA在植物中的加工调节，温度对两栖动物免疫学的影响，管状器官的发育，以及对DNA修复机制的基本理解。 使用该仪器的研究结果将在同行评议的出版物、科学会议上传播，并在该机构教师参与的整个外联活动中向社区传播。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Intratumoural haematopoietic stem and progenitor cell differentiation into M2 macrophages facilitates the regrowth of solid tumours after radiation therapy

• DOI: 10.1038/s41416-021-01652-y
• 发表时间: 2021-12-20
• 期刊: BRITISH JOURNAL OF CANCER
• 影响因子: 8.8
• 作者: Parsons，Tyler M.;Buelow，Katie L.;Madlambayan，Gerard J.
• 通讯作者: Madlambayan，Gerard J.

Identification of biomarkers indicative of functional skeletal stem cells

• DOI: 10.1111/ocr.12260
• 发表时间: 2019-05-01
• 期刊: ORTHODONTICS & CRANIOFACIAL RESEARCH
• 影响因子: 3.1
• 作者: Qiryaqoz, Zeena;Timilsina, Suraj;Villa-Diaz, Luis G.
• 通讯作者: Villa-Diaz, Luis G.